Fura-10,钾盐 货号21110-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Fura-10,钾盐

Fura-10,钾盐

Fura-10,钾盐    货号21110 货号 21110 存储条件 在零下15度以下保存, 避免光照
规格 5×50 ug 价格 1200
Ex (nm) 354 Em (nm) 524
分子量 1024.27 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:21110

产品名称:Fura-10,钾盐

规格:5×50 ug

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1024.27

溶剂:水

 

产品介绍

与Fluo-4等单波长探针不同,对于Ca2+,Fura探针的最大吸收(或荧光激发)从380 nm(Fura-2),415 nm(Fura-8和Fura-10)转变,与Ca2+结合的无螯合剂可达到约340 nm(Fura-2),355 nm(Fura-8和Fura-10),最大荧光发射的波长相对独立于Ca2+浓度。通过使用在340nm和380nm(对于Fura-2),355nm和415nm(对于Fura-8和Fura-10)进行激发并分配荧光强度来获得Ca2+依赖性荧光信号的最大动态范围在〜510 nm(Fura-2),525 nm(Fura-8和Fura-10)处检测到。根据该比率,可以使用从校准曲线得出的解离常数(Kd)估算细胞内Ca2+的水平。通过使用在两个波长下激发产生的荧光强度之比,诸如染料分布不均匀和光致漂白等因素将被最小化,因为它们应在相同程度上影响两种测量。校准溶液最初应不含重金属离子,例如锰,这可能会影响其荧光和对钙的亲和力。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Fura-10,钾盐。 

点击查看光谱

 

参考文献

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Ho, Quynh Mai and Leem, Chae Hun
Journal: Journal of visualized experiments : JoVE (2019)

Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2.
Authors: Johnson, Martin
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 163-172

Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line.
Authors: Malaiyandi, Latha M and Sharthiya, Harsh and Barakat, Ameir N and Edwards, Joshua R and Dineley, Kirk E
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2019): 951-964

A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision.
Authors: Tinning, P W and Franssen, A J P M and Hridi, S U and Bushell, T J and McConnell, G
Journal: Journal of microscopy (2018): 212-220

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-fura-2 and Analogues.
Authors: Zhang, Guangqian and Jacquemin, Denis and Buccella, Daniela
Journal: The journal of physical chemistry. B (2017): 696-705

Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury.
Authors: Ohkubo, Masato and Miyamoto, Atsushi and Shiraishi, Mitsuya
Journal: The Journal of veterinary medical science (2016): 761-7

Interferences of resveratrol with fura-2-derived fluorescence in intracellular free-Ca(2+) concentration determinations.
Authors: Santofimia-Castaño, Patricia and Salido, Gines M and Gonzalez, Antonio
Journal: Cytotechnology (2016): 1369-80

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Leem, Chae Hun
Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology (2015): 373-82

Resveratrol Interferes with Fura-2 Intracellular Calcium Measurements.
Authors: Kopp, Richard F and Leech, Colin A and Roe, Michael W
Journal: Journal of fluorescence (2014): 279-84

Resveratrol is not compatible with a Fura-2-based assay for measuring intracellular Ca²⁺ signaling.
Authors: Paudel, Ram Chandra and Kiviluoto, Santeri and Parys, Jan B and Bultynck, Geert
Journal: Biochemical and biophysical research communications (2014): 1626-30

说明书
Fura-10,钾盐.pdf

Fura-10,钾盐 货号21111-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Fura-10,钾盐

Fura-10,钾盐

Fura-10,钾盐    货号21111 货号 21111 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 3732
Ex (nm) 354 Em (nm) 524
分子量 1024.27 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:21110

产品名称:Fura-10,钾盐

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1024.27

溶剂:水

 

产品介绍

与Fluo-4等单波长探针不同,对于Ca2+,Fura探针的最大吸收(或荧光激发)从380 nm(Fura-2),415 nm(Fura-8和Fura-10)转变,与Ca2+结合的无螯合剂可达到约340 nm(Fura-2),355 nm(Fura-8和Fura-10),最大荧光发射的波长相对独立于Ca2+浓度。通过使用在340nm和380nm(对于Fura-2),355nm和415nm(对于Fura-8和Fura-10)进行激发并分配荧光强度来获得Ca2+依赖性荧光信号的最大动态范围在〜510 nm(Fura-2),525 nm(Fura-8和Fura-10)处检测到。根据该比率,可以使用从校准曲线得出的解离常数(Kd)估算细胞内Ca2+的水平。通过使用在两个波长下激发产生的荧光强度之比,诸如染料分布不均匀和光致漂白等因素将被最小化,因为它们应在相同程度上影响两种测量。校准溶液最初应不含重金属离子,例如锰,这可能会影响其荧光和对钙的亲和力。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Fura-10,钾盐。 

点击查看光谱

 

参考文献

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Ho, Quynh Mai and Leem, Chae Hun
Journal: Journal of visualized experiments : JoVE (2019)

Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2.
Authors: Johnson, Martin
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 163-172

Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line.
Authors: Malaiyandi, Latha M and Sharthiya, Harsh and Barakat, Ameir N and Edwards, Joshua R and Dineley, Kirk E
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2019): 951-964

A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision.
Authors: Tinning, P W and Franssen, A J P M and Hridi, S U and Bushell, T J and McConnell, G
Journal: Journal of microscopy (2018): 212-220

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-fura-2 and Analogues.
Authors: Zhang, Guangqian and Jacquemin, Denis and Buccella, Daniela
Journal: The journal of physical chemistry. B (2017): 696-705

Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury.
Authors: Ohkubo, Masato and Miyamoto, Atsushi and Shiraishi, Mitsuya
Journal: The Journal of veterinary medical science (2016): 761-7

Interferences of resveratrol with fura-2-derived fluorescence in intracellular free-Ca(2+) concentration determinations.
Authors: Santofimia-Castaño, Patricia and Salido, Gines M and Gonzalez, Antonio
Journal: Cytotechnology (2016): 1369-80

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Leem, Chae Hun
Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology (2015): 373-82

Resveratrol Interferes with Fura-2 Intracellular Calcium Measurements.
Authors: Kopp, Richard F and Leech, Colin A and Roe, Michael W
Journal: Journal of fluorescence (2014): 279-84

Resveratrol is not compatible with a Fura-2-based assay for measuring intracellular Ca²⁺ signaling.
Authors: Paudel, Ram Chandra and Kiviluoto, Santeri and Parys, Jan B and Bultynck, Geert
Journal: Biochemical and biophysical research communications (2014): 1626-30

说明书
Fura-10,钾盐.pdf

Fura-10,AM 货号21115-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Fura-10,AM

Fura-10,AM

Fura-10,AM    货号21115 货号 21115 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 3732
Ex (nm) 354 Em (nm) 524
分子量 1194.14 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:21115

产品名称:Fura-10,AM

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1194.14

溶剂:DMSO

 

适用仪器


荧光酶标仪  
激发: 354 nm and 415 nm
发射: 524 nm
cutoff: 475 nm
推荐孔板: 黑色透明
读取模式: 底读模式
其他仪器
FDSS, FLIPR, ViewLux, NOVOStar, ArrayScan, FlexStation, IN Cell Analyzer

 

产品介绍

在比例钙离子指示剂中,Fura-2和Indo-1是两个最受欢迎的指示剂。 但是,使用这两种钙离子指示剂仍然存在一些挑战,特别是对于活细胞。 Fura-2的紫外线激发导致快速的光致漂白。 几年前推出了Fura-8 ,将激发光移向可见光。 尽管Fura-8表现出信号/背景比率的明显改善,但它不能像Fura-2那样很好地保留在活细胞中。 Fura-10最近被引入以解决该细胞保留问题。 在没有丙磺舒的情况下,Fura 10表现出信号/背景比的明显改善。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Fura-10,AM。 

点击查看光谱

 

参考文献

Acupotomy Alleviates Energy Crisis at Rat Myofascial Trigger Points.
Authors: Zhang, Yi and Du, Ning-Yu and Chen, Chen and Wang, Tong and Wang, Li-Juan and Shi, Xiao-Lu and Li, Shu-Ming and Guo, Chang-Qing
Journal: Evidence-based complementary and alternative medicine : eCAM (2020): 5129562

High-salt intake increases TRPC3 expression and enhances TRPC3-mediated calcium influx and systolic blood pressure in hypertensive patients.
Authors: Hu, Yingru and Xia, Weijie and Li, Yingsha and Wang, Qianran and Lin, Shaoyang and Wang, Bin and Zhou, Cui and Cui, Yuanting and Jiang, Yanli and Pu, Xiaona and Wei, Xiao and Wu, Hao and Zhang, Hengshu and Zhu, Zhiming and Liu, Daoyan and Li, Zhiyong
Journal: Hypertension research : official journal of the Japanese Society of Hypertension (2020)

Histamine induces intracellular Ca2+ oscillations and nitric oxide release in endothelial cells from brain microvascular circulation.
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530

Pharmacological and genetic characterisation of the canine P2X4 receptor.
Authors: Sophocleous, Reece A and Berg, Tracey and Finol-Urdaneta, Rocio K and Sluyter, Vanessa and Keshiya, Shikara and Bell, Lachlan and Curtis, Stephen J and Curtis, Belinda L and Seavers, Aine and Bartlett, Rachael and Dowton, Mark and Stokes, Leanne and Ooi, Lezanne and Sluyter, Ronald
Journal: British journal of pharmacology (2020)

Reduced store-operated Ca2+ entry impairs mesenteric artery function in response to high external glucose in type 2 diabetic ZDF rats.
Authors: Schach, Christian and Wester, Michael and Leibl, Florian and Redel, Andreas and Gruber, Michael and Maier, Lars S and Endemann, Dierk and Wagner, Stefan
Journal: Clinical and experimental pharmacology & physiology (2020)

Rumex acetosa modulates platelet function and inhibits thrombus formation in rats.
Authors: Jeong, Dahye and Irfan, Muhammad and Lee, Dong-Ha and Hong, Seung-Bok and Oh, Jae-Wook and Rhee, Man Hee
Journal: BMC complementary medicine and therapies (2020): 98

A Na+ /Ca2+ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence.
Authors: Moretti, Chiaraluce and Trabalza, Simone and Granieri, Letizia and Caballo-Ponce, Eloy and Devescovi, Giulia and Del Pino, Alberto Marco and Ramos, Cayo and Venturi, Vittorio and van den Burg, Harrold A and Buonaurio, Roberto and Palmerini, Carlo Alberto
Journal: Molecular plant pathology (2019): 716-730

A3 receptor agonist, Cl-IBMECA, potentiate glucose-induced insulin secretion from MIN6 insulinoma cells possibly through transient Ca2+ entry.
Authors: Keyvanloo Shahrestanaki, Mohammad and Aghaei, Mahmoud
Journal: Research in pharmaceutical sciences (2019): 107-114

Accumulation of intramyocyte TRPV1-mediated calcium during heat stress is inhibited by concomitant muscle contractions.
Authors: Ikegami, Ryo and Eshima, Hiroaki and Mashio, Takuro and Ishiguro, Tomosada and Hoshino, Daisuke and Poole, David C and Kano, Yutaka
Journal: Journal of applied physiology (Bethesda, Md. : 1985) (2019): 691-698

Acidification is an Essential Process of Cold Atmospheric Plasma and Promotes the Anti-Cancer Effect on Malignant Melanoma Cells.
Authors: Schneider, Christin and Gebhardt, Lisa and Arndt, Stephanie and Karrer, Sigrid and Zimmermann, Julia L and Fischer, Michael J M and Bosserhoff, Anja-Katrin
Journal: Cancers (2019)

说明书
Fura-10,AM.pdf