ReadiView Blue 实时荧光定量 PCR 可视化染料 *200X*
| 货号 |
17300 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
500 Tests |
价格 |
1140 |
| Ex (nm) |
|
Em (nm) |
|
| 分子量 |
N/A |
溶剂 |
|
| 产品详细介绍 |
简要概述
产品介绍
ReadiView Blue 实时荧光定量 PCR 可视化染料 是一种 200X 浓缩的即用型惰性染料，可增强实时 PCR 反应的可见性，从而实现准确的移液和板装载。当按照指示使用时，染料的存在不会对 PCR 反应的性能产生不利影响，并且不会干扰 Helixyte Green-、SYBR® Green- 或其他探针的检测。它针对各种实时 PCR 混合物和试剂盒进行了优化，包括含有 ROX 的混合物以及用于 FAST 和常规反应时间的混合物。金畔生物是AAT Bioquest的中国代理商，为您提供ReadiView Blue 实时荧光定量 PCR 可视化染料 *200X*。
产品说明书
样品分析方案
操作步骤
以下方案仅作为指导，开始实验前，ReadiView Blue 实时荧光定量 PCR 可视化染料必须完全解冻至室温。
1.在主混合物中添加染料：
1.1 将 1 µL ReadiView Blue 实时荧光定量 PCR 可视化染料与包含引物、TaqMan 探针或 DNA 染料的 100 µL TAQuest qPCR Master Mix（2X master mix）混合。
1.2 彻底混合各组分，然后短暂离心以将溶液收集在管底。 储存在冰上，避光。
1.3对于 20 μL PCR 反应，将 10 μL 的 qPCR 主混合物与 10 μL 的 DNA 样本混合。 建议放在白色背景上以提高可见度。
注意ReadiView Blue 实时荧光定量 PCR 可视化染料也可能与各种预混液兼容，但应由用户验证以获得性能。
2.在 DNA 或 RNA 样品中添加染料：
2.1 将 1 µL ReadiView Blue 实时荧光定量 PCR 可视化染料与100 µL 含有 DNA 或 RNA 的样品混合
2.2 彻底混合各组分，然后短暂离心以将溶液收集在管底。 储存在冰上，避光。
2.3 对于 20 μL PCR 反应，将 10 μL 的 qPCR 主混合物与 10 μL 的 DNA 或 RNA 样品混合。 建议放在白色背景上以提高可见度。
参考文献
SYBR® Green RT-qPCR for the Universal Detection of Citrus Viroids.
Authors: Vidalakis, Georgios and Wang, Jinbo and Dang, Tyler and Rucker, Tavia and Bodaghi, Sohrab and Tan, Shi-Hua and Lavagi-Craddock, Irene and Syed, Alexandra and Uribe, Gerardo and Hsieh, Yi and Carbajal-Moreno, Joana
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 211-217
[Typing of Leishmania Species Causing Cutaneous Leishmaniasis by Sybr Green Based ITS-1 Real Time Polymerase Chain Reaction Method].
Authors: Gürses, Gülcan and Yentür Doni, Nebiye and Yıldız Zeyrek, Fadile and Yiğin, Akın
Journal: Mikrobiyoloji bulteni (2022): 326-338
Development and validation of multiplex SYBR Green real-time PCR assays for detection and molecular surveillance of four tick-borne canine haemoparasites.
Authors: Padmaja, M and Singh, Harkirat and Panwar, Harsh and Jyoti, and Singh, Niraj Kumar and Singh, Nirbhay Kumar
Journal: Ticks and tick-borne diseases (2022): 101937
Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR.
Authors: Tao, Yile and Yue, Yang and Qiu, Guangyu and Ji, Zheng and Spillman, Martin and Gai, Zhibo and Chen, Qingfa and Bielecki, Michel and Huber, Michael and Trkola, Alexandra and Wang, Qiyuan and Cao, Junji and Wang, Jing
Journal: Applied microbiology and biotechnology (2022): 2207-2218
Establishment of SYBR green I-based quantitative real-time polymerase chain reaction for the rapid detection of a novel Chaphamaparvovirus in cats.
Authors: Liu, Xunbi and Li, Shuyan and Liu, Xuan and Wang, Run and Xie, Xiangyu and Wu, Haiqiang and Wang, Yong
Journal: 3 Biotech (2022): 91
Multiplex SYBR Green real-time PCR for Lactobacillus acidophilus group species targeting biomarker genes revealed by a pangenome approach.
Authors: Kim, Eiseul and Kim, Dayoung and Yang, Seung-Min and Kim, Hae-Yeong
Journal: Microbiological research (2022): 127013
A SYBR Green-based real-time RT-PCR assay to differentiate the H1N1 influenza virus lineages.
Authors: Cong, Yulin and Sun, Yixue and Deng, Xiaoyu and Yu, Haiying and Lian, Xiaohuan and Cong, Yanlong
Journal: Journal of virological methods (2022): 114387
One-step SYBR green-based real-time RT-PCR assay for detection of foot-and-mouth disease virus circulating in India.
Authors: Biswal, Jitendra K and Jena, Biswa Ranjan and Ali, Syed Zeeshan and Ranjan, Rajeev and Mohapatra, Jajati K and Singh, Rabindra Prasad
Journal: Virus genes (2022): 113-121
Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis.
Authors: Sarkar, Shovon Lal and Alam, A S M Rubayet Ul and Das, Prosanto Kumar and Pramanik, Md Hasan Ali and Al-Emran, Hassan M and Jahid, Iqbal Kabir and Hossain, M Anwar
Journal: Scientific reports (2022): 6501
Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.
Authors: Xia, Yin-He and Shi, Zi-Cong and Wang, Xin-Wei and Li, Yong-Tao and Wang, Zeng and Chang, Hong-Tao and Liu, Hong-Ying and Chen, Lu and Wang, Chuan-Qing and Yang, Xia
Journal: Molecular and cellular probes (2021): 101730
说明书
ReadiView Blue 实时荧光定量 PCR 可视化染料 *200X*.pdf
