标签归档：human

人类α凝血因子（Human Factor αXIIa）HFXIIa 1212a说明书

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 Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Enzyme Research Laboratories为生物技术公司,制药行业、医院和大学的研究实验室提供用于的纯化抗凝因子。除了其制造能力，Enzyme Research Laboratories也创新研发领域中的血液凝固技术。

Human Factor αXIIa

 

HFXIIa 1212a             Human Factor αXIIa 
                              （Activated Hageman Factor）

Human Factor α-XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system. Human Factor XII and prekallikrein are thought to be involved in a reciprocal activation mechanism in which Factor XIIa activates prekallikrein to kallikrein, which in turn converts Factor XII to XIIa. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. ERL offers Factor α-XIIa which is activated by the autoactivation process with Dextran Sulfate and re-purified to remove the activator. The protein purity is determined by SDS-PAGE and activity is determined via clotting assay.

Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3
*Extinction Coefficient （1%） = 14.1
Molecular Weight = 80,000 daltons


 

人凝血因子XIa（Human Factor XIa）HFXIa 1111a 说明书

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 Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research ,Enzyme Research *代理,Enzyme Research 中国代理

Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Human Factor XIa

 

HFXIa 1111a             Human Factor XIa

Prepared from Human Factor XI using Human Factor Xlla. This Xlla was removed using a corn trypsin inhibitor column. Complete activation is observed by SDS-PAGE. Factor XI, through the contact factor pathway cascade, is activated to Factor XIa via Factor Xlla and High Molecular Weight Kininogen. During activation by Factor Xlla and HK, FXI undergoes proteolytic cleavage in which the Mr=80,000 chain reportedly is cleaved to a heavy and light chain with Mr of about 48,000 and 33,000. This Factor XIa is responsible for the activation of Factor IX to Factor IXa. Unlike other examples of activation of Vitamin K-dependent blood-clotting proteins, Factor XIa proteolysis of Factor IX does not require membrane surfaces.

Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3
*Extinction Coefficient （1%） = 13.1
Molecular Weight = 160,000 daltons

人类凝血因子Xa Human Factor Xa（HFXa 1011）说明书

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Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Human Factor Xa

 

HFXa 1011             Human Factor Xa

Human Factor Xa is prepared from Human Factor X by activation with Russell’s Viper Venom. This RVV-X is removed after activation. Complete activation is observed by SDS-PAGE. Factor Xa along with cofactor Va, phospholipids and calcium ions, （the prothrombinase complex） catalyzes the rapid conversion of prothrombin to thrombin.

Buffer composition = 20 mM Bis Tris/0.7 M NaCl/pH 6.0
*Extinction Coefficient （1%） =11.6
One unit = 8 µg
Molecular Weight = 46,000 daltons

Also available: Human Factor Xaβ, des-Gla Xaβ and 
Xa inactivated

人类凝血因子（Human Factor IXa β）HFIXa 1080说明书

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 Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Human Factor IXa β

 

HFIXa 1080             Human Factor IXa β

Prepared from Human Factor IX by activation with Bovine Factor XIa. This Bovine Factor XIa is removed after activation. Complete activation is observed by SDS-PAGE. The Factor XIa activates Factor IX in a two-step reaction. In the first step, an internal Arg-Ala bond is cleaved, and in the second step, an Arg-Val bond is cleaved. The second cleavage leads to the liberation of an activation peptide from the NH2-terminal portion of the heavy chain to produce Factor IXaβ.

Buffer composition = 20 mM Tris-HCl/0.1 M NaCl /pH 7.4
*Extinction Coefficient （1%） = 14.3 
One unit =5 µg
Molecular Weight = 45,000 daltons

 

人活化蛋白C（Human Activated Protein C）APC说明书

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Human Activated Protein C

 

APC             Human Activated Protein C

Activated Protein C （APC） is a serine protease derived from the two chain vitamin K dependent zymogen, Protein C. APC inhibits blood coagulation through the selective inactivation of the cofactors Va and VIIIa. APC is prepared from protein C by activation with purified thrombin. This thrombin is removed after activation by ion exchange chromatography. Activated Protein C purity is determined by SDS-PAGE and shows complete reduction upon incubation with 2-mercaptoethanol.

Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/ pH 7.4
*Extinction Coefficient （1%） = 14.5 
Molecular Weight = 56,000 daltons

Also available: des-Gla-Human Activated Protein C

 

人类凝血因子（Human Factor VIIa） HFVIIa说明书

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 Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Human Factor VIIa

 

HFVIIa             Human Factor VIIa

Prepared from purified Human Factor VII using Human Factor XIIa. The Factor Xlla is removed using affinity chromatography. Purity is determined by SDS-PAGE. Human Factor VIIa reduces to 29,500 and 23,500 with the addition of 2-mercaptoethanol. Activity is determined via clotting assay.  Factor Vlla, in the presence of calcium ions and Tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa.

Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4
*Extinction Coefficient （1%） = 13.9
Molecular Weight = 50,000 daltons

Also available: des-Gla-Human Factor VIIa and 
Factor VIIa inactivated

人类γ凝血酶Human γ-Thrombin HGT 说明书

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Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。

Human γ-Thrombin

 

HGT              Human γ-Thrombin

A non-clotting derivative thrombin produced from Humanα-Thrombin by controlled incubation with Trypsin-Sepharose. Gamma-thrombin is a noncoagulant form of thrombin that retains much of its plaet-activating capacity. Gamma-thrombin purity is determined by SDS-PAGE.
 

Buffer Composition = 10 mM Sodium Acetate/0.75 M NaCl/pH 6.0
Molecular Weight = 12,000 daltons
Concentration determined by BCA

人类α凝血酶（Human α-Thrombin）HT 1002a 说明书

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 Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research ,Enzyme Research *代理,Enzyme Research 中国代理
Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Human α-Thrombin

 

HT 1002a              Human α-Thrombin （Factor IIa）

Prepared from homogeneous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. Human Thrombin purity is determined by SDS-PAGE. This activated enzyme has a minimum activity of 2,700 NIH units/mg when compared to NIH standard thrombin.

Buffer composition = 50 mM Sodium Citrate/0.2 M NaCl/0.1% PEG-8000/pH 6.5
*Extinction Coefficient （1%） = 18.3 
Molecular Weight = 37,000 daltons


人类赖氨酸型纤溶酶原Human Lys-Plasminogen LPg 2002 说明书

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Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Human Lys-Plasminogen

 

LPg 2002          Human Lys-Plasminogen

Purified from homogeneous glu-plasminogen by activation with Plasmin. This activation results in the release of a 76 amino acid residue peptide （Glu-Lys76）. This Lys77-Plasminogen can be readily converted to Lys77-Plasmin by any of the common plasminogen activators. The protein purity is determined by SDS-PAGE.

Buffer composition =50 mM Tris-HCl/0.1 M NaCl /pH 7.4
*Extinction Coefficient （1%） = 17.0
Molecular Weight = 83,000 daltons

人激肽释放酶原（Human Prekallikrein） HPK 1302 说明书

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 Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research ,Enzyme Research *代理,Enzyme Research 中国代理

Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。

Human Prekallikrein

 

HPK 1302          Human Prekallikrein

Purified from fresh frozen human plasma. Human Prekallikrein is a single chain gamma globulin glycoprotein that participates in the early phase of contact activation, kinin formation and fibrinolysis. Prekallikrein purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay.

Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3
*Extinction Coefficient （1%） =11.7
Molecular Weight = 86,000 daltons

 

人凝血因子Human Factor XII （HFXII 1212）说明书

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 Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research ,Enzyme Research *代理,Enzyme Research 中国代理

Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。
Enzyme Research Laboratories为生物技术公司,制药行业、医院和大学的研究实验室提供用于的纯化抗凝因子。除了其制造能力，Enzyme Research Laboratories也创新研发领域中的血液凝固技术。

Human Factor XII

 

HFXII 1212         Human Factor XII （Hageman Factor）

Purified from fresh frozen human plasma. Human Factor XII is part of the contact activation system. Human Factor XII is a single chain glycoprotein with a molecular weight of about 80,000. Once activated, principally from the action of kallikrein, Factor XII is converted into an active serine protease （Factor α-Xlla） that functions in the in vivo initiation of blood coagulation, fibrinolysis, and kinin formation. Human Factor XII has been purified by ion exchange chromatography. Protein purity is determined by SDS-PAGE and activity is determined via clotting assay.

Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3 
*Extinction Coefficient （1%） =14.1
Molecular Weight = 80,000 daltons

人凝血因子（Human Factor XIII）HFXIII 1313说明书

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Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

 

Human Factor XIII

 

HFXIII 1313         Human Factor XIII （Fibrin Stabilizing Factor）

Human Factor XIII is a tetramer composed of two pairs of chains held together by noncovalent bonds. After activation of the zymogen via Thrombin to its active enzyme form, Factor XIIIa is responsible for catalyzing the formation of covalent bridges between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is very insoluble and resistant to lysis.

Buffer composition =50 mM Tris-HCl/0.1 M NaCl/1 mM EDTA/10u/mL aprotinin/20% glycerol/pH 7.5
*Extinction Coefficient （1%）=13.8
Molecular Weight = 320,000 daltons

 

 

 

人凝血因子XI（Human Factor XI）HFXI 1111 说明书

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 Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

 

Human Factor XI

 

HFXI 1111             Human Factor XI                                                         （Plasma Thromboplastin Antecedent）

Prepared from fresh frozen human plasma. Human Factor XI is a two-chain glycoprotein with molecular weight of 160,000 daltons.  The two chains are identical disulfide bonded polypeptides with molecular weights of 80,000 daltons.  Factor XI is activated to Factor XIa by Factor Xlla. Purity of Factor XI is assessed by SDS-PAGE.  Activity is determined by clotting assay.

Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCI/pH 5.3
*Extinction Coefficient （1%） = 13.1
Molecular Weight = 160,000 daltons

 

人类凝血因子（Human Factor X）HFX 1010说明书

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Enzyme Research Laboratories为生物技术公司,制药行业、医院和大学的研究实验室提供用于的纯化抗凝因子。除了其制造能力，Enzyme Research Laboratories也创新研发领域中的血液凝固技术。

 

Human Factor X

 

HFX 1010         Human Factor X               （Stuart Prower Factor）

Prepared from fresh frozen human plasma. The purity of this Vitamin K dependent protein is determined by SDS-PAGE  and shows total reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay. Human Factor X, once activated via the Contact Factor Pathway or the Tissue Factor Pathway, is responsible for the conversion of Prothrombin to Thrombin.

Buffer composition 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4
*Extinction Coefficient （1%） =11.6
One unit =8 µg
Molecular Weight =58,800 daltons

 

人类凝血因子IX Human Factor IX （HFIX 1009）说明书

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 Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research ,Enzyme Research *代理,Enzyme Research 中国代理
Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

 

Human Factor IX

 

HFIX 1009              Human Factor IX （Christmas Factor）

Prepared from fresh frozen human plasma. This protein purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.  Activity is determined via clotting assay.  Human Factor IX, activated by either the Contact or Tissue Factor Pathway, is responsible for the activation of Factor X to Xa.

Buffer composition: 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4
*Extinction Coefficient （1%） = 13.2
One unit = 5 µg
Molecular Weight = 56,000 daltons

 

 

 

人体蛋白C（Human Protein C）HPC 1001 说明书

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 Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Enzyme Research Laboratories为生物技术公司,制药行业、医院和大学的研究实验室提供用于的纯化抗凝因子。除了其制造能力，Enzyme Research Laboratories也创新研发领域中的血液凝固技术。

Human Protein C

 

HPC 1001              Human Protein C

Purified from fresh frozen human plasma using a combination of salt precipitations and column chromatography. The protein purity is determined by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Protein C is activated to the serine protease, Activated Protein C （APC）, by a-thrombin or the complex of a-thrombin/thrombomodulin and is a potent anticoagulant through the selective inactivation of Factors Va and VIIIa.

Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/1 mM Benzamidine/pH 7.4
*Extinction Coefficient （1%） = 14.5
Molecular Weight = 62,000 daltons

Also available: des-Gla-Human Protein C

 

人凝血因子X-GD（Human Factor X-GD）HFX-GD 说明书

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 Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research ,Enzyme Research *代理,Enzyme Research 中国代理

Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。

Human Factor X-GD

 

HFX-GD       Human Factor X Des-Gla               

Factor X- gla domainless was prepared from plasma purified Human Factor X using alpha-chymotrypsin.  The alpha-chymotrypsin and all other products were removed using an anion exchange column after digestion.  Purity is >95% by SDS-PAGE.  Protein concentration is determined by BCA. 

Buffer composition 100 mM Tris-HCl/50 mM NaCl /pH 7.5
Molecular Weight ~57,300 daltons

人凝血因子Ⅶ（Human Factor VII） HFVII 1007 说明书

发表于由

 Enzyme Research Laboratories为生物技术公司,制药行业、医院和大学的研究实验室提供用于的纯化抗凝因子。除了其制造能力，Enzyme Research Laboratories也创新研发领域中的血液凝固技术。

Enzyme Research的产品线包括:
从人类和牛等离子体纯化酶原激活酶
凝血因子的单克隆和多克隆抗体
检测凝血因子ELISA试剂
自定义的肽和蛋白质纯化服务

Human Factor VII

 

HFVII 1007              Human Factor VII （Proconvertin）

Prepared from fresh frozen human plasma. Human Factor VII is a single-chain vitamin K dependent glycoprotein found in trace quantities in plasma （0.5 mg/Liter）. In the Tissue Factor Pathway of coagulation, Human Factor Vlla, in the presence of calcium ions and tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa.

Buffer composition =20 mM Tris-HCl/0.1 M NaCl/1 mM Benzamidine/pH 7.4
*Extinction Coefficient （1%） = 13.9
Molecular Weight = 50,000 daltons

上海金畔生物科技有限公司

人凝血酶原Human Prothrombin （Factor II） HP 1002

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 Enzyme Research （http://www.enzymeresearch.co.uk/）是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Enzyme Research Laboratories为生物技术公司,制药行业、医院和大学的研究实验室提供用于的纯化抗凝因子。除了其制造能力，Enzyme Research Laboratories也创新研发领域中的血液凝固技术。

Human Prothrombin

 

HP 1002               Human Prothrombin （Factor II）

Prepared from fresh frozen human plasma. Human Prothrombin is a glycoprotein of molecular weight 72,000, and consists of a single polypeptide chain. Activation of Prothrombin by Factor Va, Xa and phospholipids yields the serine protease Thrombin. Prothrombin purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.

Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4 
*Extinction Coefficient （1%） = 13.6
One unit = 90 µg
Molecular Weight =72,000 daltons

Also available: des-Gla-Human Prothrombin and Prothrombin Fragment 1 and 2