α-凝血酶α-Thrombin选购指南

α-凝血酶α-Thrombin选购指南

凝血酶Thrombin (Factor IIa)是一种丝氨酸蛋白酶,也是血液凝血级联反应中的主要效应蛋白酶,显现出促凝和抗凝的特性。凝血酶是由在凝血酶原复合物中的非活性凝血酶原在Xa因子(FXa)因子的作用下通过蛋白裂解的方式产生

【生理作用】当循环凝血因子在暴露的血管外组织与组织因子相接触时,凝血酶会在组织上聚集。凝血酶通过激活血小板,催化纤维蛋白原转化为纤维蛋白,促进血稳定而在血栓性疾病的引发和传播上有着核心作用

α-凝血酶(α-Thrombin)是一种高度特异性的丝氨酸蛋白酶,由凝血酶原(prothrombinII因子)经蛋白水解活化而来。它在凝血过程中起着非常重要的作用,不仅能够促使纤维蛋白凝块的产生,还负责提供反馈信号来激活前辅因子:V因子和VIII因子。也可以激活XIII因子和血小板,或者用作血管收缩剂。

在体内,活化的X因子(Factor Xa)切割凝血酶原,释放出活性肽并将凝血酶切割成具有催化活性的α-凝血酶。α-凝血酶由一条轻链(A chain)(Mw ~6,000)和一条重链(B chain)(Mw ~31,000)通过二硫键连接而成。某些情况下,α-凝血酶会发生自溶生成β-凝血酶和γ-凝血酶。β-凝血酶由对α-凝血酶A链水解并切割含有B链糖基化位点的小片段(B1B2)组合而成。

除了用于凝血研究之外,α-凝血酶还常用来位点特异性切割融合蛋白。基因重组时将凝血酶识别位点插入在目的蛋白与利于后续纯化和/或表达的多肽或者蛋白之间,通过凝血酶切割表达的重组子即可释放目的蛋白。凝血酶本身可通过亲和层析技术快速去除。

作为生物活性物质的凝血酶:是一种在人体抗凝,促凝的凝血级联反应中有着重要作用的生物活性蛋白。

产品性质

中文别名Chinese synonym

α-凝血酶;IIa 因子;

英文别名(English synonym

α-Thrombin; Factor IIa;

CAS号(CAS NO.

9002-04-4

分子量(Molecular weight

37000 daltons

活力(Activity

2700.00 NIH U/mg

缓冲液组分(Buffer

50mM Sodium Citrate/0.2 M NaCl/0.1% PEG-8000/pH 6.5

消光系数(Extinction coefficient)(1%

18.3

运输和保存方法

粉末冰袋运输,2-8保存。液体干冰运输,≤-60保存

凝血酶Thrombin (Factor IIa)  抗体

11571-05021 Thrombin, anti-human 150 ug 2800 AssayPro
11571-05011 Thrombin, anti-human 150 ug 2320 AssayPro
BP104C Bovine anti-Thrombin (AT) 1 x 10 mg 4545 hyphen biomed
60130-05021 alpha-Thrombin (Factor IIa), anti-human 150 ug 2320 AssayPro
60130-05011 alpha-Thrombin (Factor IIa), anti-human 150 ug 2000 AssayPro
SAHT-AP Anti-human Thrombin (F.IIa) sheep, AP IgG, 0.5 mg 5168 Enzyme Research2016
PAHT-S Sheep anti-Human  Thrombin 1×1 mg 436.8 Haematologic Technologies
PAHT-S Sheep anti-Human  Thrombin 1×5 mg 1506.96 Haematologic Technologies
SART-IG Sheep anti  rabbit Thrombin  purified IgG 10 mg 2414 affinitybiologicals
SART-HRP Sheep anti  rabbit Thrombin  peroxidase conjugated IgG 0.2 mg 4131 affinitybiologicals
SART-AP Sheep anti  rabbit Thrombin  affinity purified IgG 0.5 mg 5304 affinitybiologicals
SAHT-IG Sheep anti  human Thrombin  purified IgG 10 mg 2227 affinitybiologicals
SAHT-HRP Sheep anti  human Thrombin  peroxidase conjugated IgG 0.2 mg 4029 affinitybiologicals
SAHT-APFTC Sheep anti  human Thrombin  affinity purified, FITC conjugated IgG 0.1 mg 4029 affinitybiologicals
SAHT-APBIO Sheep anti  human Thrombin  affinity purified, Biotinylated IgG 0.1 mg 4029 affinitybiologicals
SAHT-AP Sheep anti  human Thrombin  affinity purified IgG 0.5 mg 5168 affinitybiologicals
SABT-IG Sheep anti  bovine Thrombin  purified IgG 10 mg 2227 affinitybiologicals
SABT-HRP Sheep anti  bovine Thrombin  peroxidase conjugated IgG 0.2 mg 4029 affinitybiologicals
SABT-AP Sheep anti  bovine Thrombin  affinity purified IgG 0.5 mg 5168 affinitybiologicals
SART-IG Anti-rabbit Thrombin (rF.IIa)sheep, purified IgG,                                 10 mg 10 mg 2600 Enzyme Research2016
SART-HRP Anti-rabbit Thrombin (rF.IIa)sheep, perox-conj. IgG, 0.2 mg 4131 Enzyme Research2016
SART-AP Anti-rabbit Thrombin (rF.IIa)sheep, affinity purified IgG, 0.5 mg 5304 Enzyme Research2016
SATAFI-AP Anti-human Thrombin Activatable Fibrinolysis Inhibitor (TAFI)sheep, affinity purified IgG, 0.5 mg 5066 Enzyme Research2016
SAHT-HRP Anti-human Thrombin (F.IIa)sheep, perox-conj. IgG, 0.2 mg 4029 Enzyme Research2016
SAHT-IG Anti-human Thrombin (F.IIa) sheep, purified IgG, 10 mg 2600 Enzyme Research2016
SAHT-APFTC Anti-human Thrombin (F.IIa) sheep, AP-FITC 0.1 mg 4029 Enzyme Research2016
AHT-5020 Anti-Human Thrombin 1×100 µg 1179.36 Haematologic Technologies
AHT-5020 Anti-Human Thrombin 5×100 µg 3057.6 Haematologic Technologies
SABT-IG Anti-bovine Thrombin (bF.IIa)sheep, purified IgG, 10 mg 2600 Enzyme Research2016
SABT-HRP Anti-bovine Thrombin (bF.IIa)sheep, perox-conj. IgG, 0.2 mg 4029 Enzyme Research2016
SABT-AP Anti-bovine Thrombin (bF.IIa)sheep, affinity purified IgG, 0.5 mg 5168 Enzyme Research2016
SATAFI-IG  Anti-human Thrombin Activatable Fibrinolysis Inhibitor (TAFI)sheep, purified IgG, 10 mg 2635 Enzyme Research2016

 

凝血酶Thrombin (Factor IIa)  试剂盒

货号 品名 包装 价格 品牌
ET4010-1 Human Thrombin ELISA Kit 96 well plate 4760 AssayPro
Thrombin-HCII Complex Thrombin-HCII Complex elisa kit 5 plate 8024 Enzyme Research2016
ADG844 OLIGOBIND® Thrombin activity assay 96 microwell plate 9200 American Diagnostica Inc

凝血酶Thrombin (Factor IIa)  抑制剂

货号 品名 包装 价格 品牌
TAFI-01 Thrombin-Activatable   Fibrinolysis Inhibitor 1×50 µg 4298.83272 Haematologic Technologies
TAFI-01 Thrombin-Activatable   Fibrinolysis Inhibitor 1×1 mg 68592.36384 Haematologic Technologies
TAFI-01 Thrombin-Activatable   Fibrinolysis Inhibitor 5×50 µg 19250.2674 Haematologic Technologies
TAFI-01 Thrombin-Activatable   Fibrinolysis Inhibitor 5×1 mg 308594.7774 Haematologic Technologies
TAFI-01 Thrombin-Activatable   Fibrinolysis Inhibitor 10×1 mg 555305.2596 Haematologic Technologies
CK002L HEMOCLOT Thrombin Inhibitors 3×2.5 mL 2130 hyphen biomed
CK002K HEMOCLOT Thrombin Inhibitors 3×1 mL 1110 hyphen biomed

凝血酶Thrombin (Factor IIa)  发色底物

SN-17a D-FPR-ANSNH-C6H11 • 2 HCl 100×1 mg 184235.688 Haematologic Technologies
SN-59 D-VPR-ANSNH-C4H9 • 2 HCl 1×1 mg 1842.35688 Haematologic Technologies
229005 BIOPHEN CS-01(81) – Thrombin Chromogenic Substrate 1 x 25 mg 3000 hyphen biomed
229001 BIOPHEN CS-01(38) – Thrombin Chromogenic substrate 1 x 25 mg 3000 hyphen biomed
0082032439 S-2238   H-D-Phe-Pip-Arg-pNA•2HCl  25mg 3500 Chromogenix2014
SN-20 Boc-L-FPR-ANSNH-C2H5 1×1 mg 1842.35688 Haematologic Technologies

凝血酶Thrombin (Factor IIa) ?其他耗材和试剂

ADG844T50 Thrombin blood collection tubes, 3 ml 50 tubes 5525 American Diagnostica Inc
ADG844T25 Thrombin blood collection tubes, 3 ml 25 tubes 3025 American Diagnostica Inc
CK011L HEMOCLOT Thrombin Time (T.T.) 6 x 8 mL 915 hyphen biomed
CK011K HEMOCLOT Thrombin Time (T.T.) 6 x 2 mL 285 hyphen biomed
CK001L HEMOCLOT Thrombin (human) 6 x 8 mL 1155 hyphen biomed
CK001K HEMOCLOT Thrombin (human) 6 x 2 mL 420 hyphen biomed

凝血酶Thrombin (Factor IIa)  蛋白

货号 品名 包装 价格 品牌
PT62082 Thrombin, Mouse 50 ug 3900 AssayPro
PP004K Anti-Thrombin (AT) 6 x 150 µg 3030 hyphen biomed
PP004B Anti-Thrombin (AT) 1 x 1.5 mg 1545 hyphen biomed
PP004A Anti-Thrombin (AT) 1 x 150 µg 720 hyphen biomed
HT 1002a Human a-Thrombin Priced per 1000 units 3,000+ units 2600 Enzyme Research2016
HT 1002a Human a-Thrombin Priced per 1000 units <3,000 units 2600 Enzyme Research2016
BT 1002a Bovine Thrombin (Priced per 1000 units) <3,000  units     2600 Enzyme Research2016
RIIa Rat Thrombin 0.1 mg 5199.9192 Enzyme Research2016
RbIIa Rabbit Thrombin 0.1 mg 5199.9192 Enzyme Research2016
PIIa Porcine Thrombin 0.1 mg 5199.9192 Enzyme Research2016
MIIa Murine Thrombin 0.05 mg 2600 Enzyme Research2016
MCT-5020 Mouse Thrombin 1×50 µg 4004.1456 Haematologic Technologies
MCT-5020 Mouse Thrombin 5×50 µg 17771.208 Haematologic Technologies
MCT-5020 Mouse Thrombin 1×1 mg 63116.34 Haematologic Technologies
MCT-5020 Mouse Thrombin 25×1 mg 1277277.456 Haematologic Technologies
MCT-5020 Mouse Thrombin 5×1 mg 283889.424 Haematologic Technologies
HTi   Human Thrombin Inactivated 1.0 mg 2600 Enzyme Research2016
EZ006O Human Thrombin 1 x 10 NIH 195 hyphen biomed
EZ006K Human Thrombin 6 x 10 NIH 825 hyphen biomed
EZ006B Human Thrombin 1 x 1000 NIH 3765 hyphen biomed
HCGT-0021 Human gamma-thrombin 1×100 µg 992.03832 Haematologic Technologies
HCGT-0021 Human gamma-thrombin 5×100 µg 4487.7924 Haematologic Technologies
HCGT-0021 Human gamma-thrombin 5×1 mg 38736.7344 Haematologic Technologies
HCGT-0021 Human gamma-thrombin 10×1 mg 76292.4708 Haematologic Technologies
HCGT-0021 Human gamma-thrombin 50×1 mg 343670.418 Haematologic Technologies
HCGT-0021 Human gamma-thrombin 100×1 mg 670806.864 Haematologic Technologies
HCGT-0021 Human gamma-thrombin 500×1 mg 3035164.86 Haematologic Technologies
HCBT-0022 Human beta-thrombin 1×100 µg 992.03832 Haematologic Technologies
HCBT-0022 Human beta-thrombin 5×100 µg 4487.7924 Haematologic Technologies
HCBT-0022 Human beta-thrombin 5×1 mg 38736.7344 Haematologic Technologies
HCBT-0022 Human beta-thrombin 10×1 mg 76292.4708 Haematologic Technologies
HCBT-0022 Human beta-thrombin 50×1 mg 343670.418 Haematologic Technologies
HCBT-0022 Human beta-thrombin 100×1 mg 670806.864 Haematologic Technologies
HCBT-0022 Human beta-thrombin 500×1 mg 3035164.86 Haematologic Technologies
HCT-FPRCK Human alpha-thrombin-FPRCK 1×100 µg 850.31856 Haematologic Technologies
HCT-FPRCK Human alpha-thrombin-FPRCK 1x1mg 5172.77124 Haematologic Technologies
HCT-FPRCK Human alpha-thrombin-FPRCK 5×100 µg 3542.994 Haematologic Technologies
HCT-FPRCK Human alpha-thrombin-FPRCK 5x1mg 25509.5568 Haematologic Technologies
HCT-FPRCK Human alpha-thrombin-FPRCK 10x1mg 46295.1216 Haematologic Technologies
HCT-FPRCK Human alpha-thrombin-FPRCK 50x1mg 226751.616 Haematologic Technologies
HCT-FPRCK Human alpha-thrombin-FPRCK 100x1mg 403901.316 Haematologic Technologies
HCT-DFP Human alpha-thrombin-DFP 1×100 µg 850.31856 Haematologic Technologies
HCT-DFP Human alpha-thrombin-DFP 1×1 mg 5172.77124 Haematologic Technologies
HCT-DFP Human alpha-thrombin-DFP 5×100 µg 3542.994 Haematologic Technologies
HCT-DFP Human alpha-thrombin-DFP 5×1 mg 25509.5568 Haematologic Technologies
HCT-DFP Human alpha-thrombin-DFP 10×1 mg 46295.1216 Haematologic Technologies
HCT-DFP Human alpha-thrombin-DFP 50×1 mg 226751.616 Haematologic Technologies
HCT-DFP Human alpha-thrombin-DFP 100×1 mg 403901.316 Haematologic Technologies
HCT-BFPRCK Human alpha-thrombin-BFPRCK 1×100 µg 850.31856 Haematologic Technologies
HCT-BFPRCK Human alpha-thrombin-BFPRCK 1×1 mg 5172.77124 Haematologic Technologies
HCT-BFPRCK Human alpha-thrombin-BFPRCK 5×100 µg 3542.994 Haematologic Technologies
HCT-BFPRCK Human alpha-thrombin-BFPRCK 5×1 mg 25509.5568 Haematologic Technologies
HCT-BFPRCK Human alpha-thrombin-BFPRCK 10×1 mg 46295.1216 Haematologic Technologies
HCT-BFPRCK Human alpha-thrombin-BFPRCK 50×1 mg 226751.616 Haematologic Technologies
HCT-BFPRCK Human alpha-thrombin-BFPRCK 100×1 mg 403901.316 Haematologic Technologies
HCT-0020 Human alpha-thrombin 1×100 µg 698.88 Haematologic Technologies
HCT-0020 Human alpha-thrombin 1×1 mg 2140.32 Haematologic Technologies
HCT-0020 Human alpha-thrombin 5×100 µg 2948.4 Haematologic Technologies
HCT-0020 Human alpha-thrombin 5×1 mg 9172.8 Haematologic Technologies
HCT-0020 Human alpha-thrombin 10×1 mg 16816.8 Haematologic Technologies
HCT-0020 Human alpha-thrombin 50×1 mg 81900 Haematologic Technologies
HCT-0020 Human alpha-thrombin 100×1 mg 146328 Haematologic Technologies
HCT-0020 Human alpha-thrombin 500×1 mg 677040 Haematologic Technologies
HGT H. Gamma Thrombin 1.0 mg 6827.1048 Enzyme Research2016
CIIa Canine Thrombin 0.1 mg 5199.9192 Enzyme Research2016
BT 1002a Bovine Thrombin (Priced per 1000 units) 3,000+ units 2600 Enzyme Research2016
BE501K Bovine Thrombin 10 x 500 NIH 14850 hyphen biomed
BE501C Bovine Thrombin 1 x 500 NIH 1875 hyphen biomed
BE102K Bovine Thrombin 6 x 21 NIH 885 hyphen biomed
BE102C Bovine Thrombin 1 x 1000 NIH 3030 hyphen biomed
BE102B Bovine Thrombin 1 x 100 NIH 735 hyphen biomed
BE102A Bovine Thrombin 1 x 21 NIH 195 hyphen biomed
BCT-FPRCK Bovine alpha-thrombin-FPRCK 1×200 µg 992.03832 Haematologic Technologies
BCT-FPRCK Bovine alpha-thrombin-FPRCK 1×1 mg 3377.65428 Haematologic Technologies
BCT-FPRCK Bovine alpha-thrombin-FPRCK 5×200 µg 4015.3932 Haematologic Technologies
BCT-FPRCK Bovine alpha-thrombin-FPRCK 5×1 mg 16652.0718 Haematologic Technologies
BCT-FPRCK Bovine alpha-thrombin-FPRCK 10×1 mg 29761.1496 Haematologic Technologies
BCT-FPRCK Bovine alpha-thrombin-FPRCK 50×1 mg 145262.754 Haematologic Technologies
BCT-FPRCK Bovine alpha-thrombin-FPRCK 100×1 mg 266905.548 Haematologic Technologies
BCT-DFP Bovine alpha-thrombin-DFP 1×200 µg 992.03832 Haematologic Technologies
BCT-DFP Bovine alpha-thrombin-DFP 1×1 mg 3377.65428 Haematologic Technologies
BCT-DFP Bovine alpha-thrombin-DFP 5×200 µg 4015.3932 Haematologic Technologies
BCT-DFP Bovine alpha-thrombin-DFP 5×1 mg 16652.0718 Haematologic Technologies
BCT-DFP Bovine alpha-thrombin-DFP 10×1 mg 29761.1496 Haematologic Technologies
BCT-DFP Bovine alpha-thrombin-DFP 50×1 mg 145262.754 Haematologic Technologies
BCT-DFP Bovine alpha-thrombin-DFP 100×1 mg 266905.548 Haematologic Technologies
BCT-BFPRCK Bovine alpha-thrombin-BFPRCK 1×200 µg 992.03832 Haematologic Technologies
BCT-BFPRCK Bovine alpha-thrombin-BFPRCK 1×1 mg 3377.65428 Haematologic Technologies
BCT-BFPRCK Bovine alpha-thrombin-BFPRCK 5×200 µg 4015.3932 Haematologic Technologies
BCT-BFPRCK Bovine alpha-thrombin-BFPRCK 5×1 mg 16652.0718 Haematologic Technologies
BCT-BFPRCK Bovine alpha-thrombin-BFPRCK 10×1 mg 29761.1496 Haematologic Technologies
BCT-BFPRCK Bovine alpha-thrombin-BFPRCK 50×1 mg 145262.754 Haematologic Technologies
BCT-BFPRCK Bovine alpha-thrombin-BFPRCK 100×1 mg 266905.548 Haematologic Technologies
BCT-1020 Bovine alpha-thrombin 1×200 µg 786.24 Haematologic Technologies
BCT-1020 Bovine alpha-thrombin 1×1 mg 2140.32 Haematologic Technologies
BCT-1020 Bovine alpha-thrombin 5×200 µg 3494.4 Haematologic Technologies
BCT-1020 Bovine alpha-thrombin 5×1 mg 9172.8 Haematologic Technologies
BCT-1020 Bovine alpha-thrombin 10×1 mg 16816.8 Haematologic Technologies
BCT-1020 Bovine alpha-thrombin 50×1 mg 81900 Haematologic Technologies
BCT-1020 Bovine alpha-thrombin 100×1 mg 146328 Haematologic Technologies
BCT-1020 Bovine alpha-thrombin 500×1 mg 677040 Haematologic Technologies
0081079639 AT III 1x25IU 1x25IU 5500 Chromogenix2014
0082072039 AT III 10×10 IU 10×10 IU 14780 Chromogenix2014
470HT alpha-thrombin, human 2,500 unit 5628

American Diagnostica Inc

 

 

haemtech Thrombin (alpha) 说明书

haemtech Thrombin (alpha) 说明书

世界*实验材料供应商 Haematologic Technologies上海金畔生物为其中国代理, Haematologic Technologies在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Haematologic Technologies就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

 Haematologic Technologies中国代理, Haematologic Technologies上海代理, Haematologic Technologies北京代理,Haematologic Technologies广东代理, Haematologic Technologies江苏代理Haematologic Technologies湖北代理,Haematologic Technologies天津,Haematologic Technologies黑龙江代理,Haematologic Technologies内蒙古代理,Haematologic Technologies吉林代理,Haematologic Technologies福建代理,Haematologic Technologies江苏代理, Haematologic Technologies浙江代理, Haematologic Technologies四川代理,

 

Haematologic Technologies Inc. (HTI) 公司是一家专门致力于高质量的人血浆蛋白的分离和特性研究的重要生产商, 其产品非常适用于体内研究。在公司发展的第二个十年里,HTI将目光投向参与血液凝集和纤维蛋白溶解,以及骨代谢调节的蛋白的研究。HTI产品系列含有超 过100种高纯度和特异性的蛋白,包括:酶原、酶、协同因子和抑制剂,同时还提供补体系列的单克隆和多克隆抗体。另外,HTI还提供一些技术服务,如:蛋 白定制、纯化、修饰、科研合作等。

 

Thrombin (alpha)

凝血酶
蛋白水解激活丝氨酸蛋白酶α-凝血酶是通过酶原,凝血酶原的蛋白水解活化产生的。酶复合物凝血酶原酶催化凝血酶原中两个肽键的蛋白水解,其产生NH2-末端衍生的F1.2区和异源二聚体α-凝血酶。α-凝血酶由通过单个二硫键与“B”链(Mr = 31,000)共价连接的“A”链(Mr = 6000)组成。

 

 

 

 

 

 

货号:HCT-0020 

Human alpha-Thrombin

Sizes 100 µg, 1 mg, 10 mg (1 mg vials)
Formulation 50% glycerol/water (v/v)
Storage -20°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:HCT-DFP 

Human alpha-Thrombin, DFP active site blocked

Size 100 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:HCT-FPRCK 

Human alpha-Thrombin, PPACK (FPRck) active site blocked

 

Size 100 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:HCT-BFPRCK 

Human alpha-Thrombin, Biotinylated PPACK (FPRck) active site blocked

Size 100 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:BCT-1020 

Bovine alpha-Thrombin

 

Sizes 200 µg, 1 mg
Formulation 50% glycerol/water (v/v)
Storage -20°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

货号:BCT-DFP 

Bovine alpha-Thrombin, DFP active site blocked

 

Size 200 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:BCT-FPRCK 

Bovine alpha-Thrombin, PPACK (FPRck) active site blocked

 

Size 200 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:BCT-BFPRCK 

Bovine alpha-Thrombin, Biotinylated PPACK (FPRck) active site blocked

 

Size 200 µg
Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4
Storage -80°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

货号:MCT-5020 

Mouse alpha-Thrombin

 

Size 50 µg
Formulation 50% glycerol/water (v/v)
Storage -20°C
Purity >95% by SDS-PAGE
Activity Determination Fibrinogen clotting or chromogenic assay
Shelf Life (properly stored) 12 months

 

 

 

 

 

 

 

凝胶 Novex 4-12%Bis-Tris
加载 人类β和γ凝血酶,每泳道1μg
缓冲 MES
标准 见蓝皮书2; 肌球蛋白(188kDa),磷酸化酶B(98kDa),BSA(62kDa),谷氨酸脱氢酶(49kDa),醇脱氢酶(38kDa),碳酸酐酶(28kDa),肌红蛋白红(17kDa),溶菌酶kDa),抑肽酶(6kDa),胰岛素,B链(3kDa)。

 

 

technoclone Thrombin Generation产品简介

technoclone Thrombin Generation产品简介

 

technoclone Thrombin Generation产品简介

 

Thrombin Generation

 

凝血酶的产生

TECHNOTHROMBIN®TGA CAL套件

校准设置为TECHNOTHROMBIN ® TGA试剂。

1 x 3 ml TGA缓冲液
1 x 0.5 ml TGA凝血酶校准物

参考 包装尺寸
5006345 1套

TECHNOTHROMBIN®TGA控制高

TGA高度控制。人血浆中凝血酶产生增加

参考 包装尺寸
5006320 5 x 1毫升

TECHNOTHROMBIN®TGA控制低

TGA低控制。人血浆中凝血酶生成减少

参考 包装尺寸
5006330 5 x 1毫升

Bovine α-Thrombin (Factor IIa)牛α-凝血酶 BT 1002a 说明书

 Enzyme Research 是一家拥有超过25年的酶研究的实验室,生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质,赢得了世界各地凝血研究实验室的信任。

Bovine α-Thrombin (Factor IIa)

 

BT 1002a            Bovine α-Thrombin (Factor IIa)

 

Prepared from bovine prothrombin through activation via the prothrombinase complex (FXa, FVa, PL and Ca++). Bovine α-Thrombin purity is >95% by SDS-PAGE. This activated enzyme has a minimum activity of 1,800 NIH units/mg when compared to NIH standard human thrombin.

Buffer composition = 50 mM Sodium Citrate/0.2 M NaCl/0.1% PEG-8000/pH 6.5
*Extinction Coefficient (1%) = 19.5
Molecular Weight= 37,000 daltons

 

人类γ凝血酶Human γ-Thrombin HGT 说明书

 Enzyme Research ,Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research ,Enzyme Research *代理,Enzyme Research 中国代理

Enzyme Research 是一家拥有超过25年的酶研究的实验室,生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。

Human γ-Thrombin

 

HGT              Human γ-Thrombin

A non-clotting derivative thrombin produced from Humanα-Thrombin by controlled incubation with Trypsin-Sepharose. Gamma-thrombin is a noncoagulant form of thrombin that retains much of its plaet-activating capacity. Gamma-thrombin purity is determined by SDS-PAGE.
 

Buffer Composition = 10 mM Sodium Acetate/0.75 M NaCl/pH 6.0
Molecular Weight = 12,000 daltons
Concentration determined by BCA

人类α凝血酶(Human α-Thrombin)HT 1002a 说明书

 Enzyme Research ,Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research ,Enzyme Research *代理,Enzyme Research 中国代理
Enzyme Research 是一家拥有超过25年的酶研究的实验室,生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质,赢得了世界各地凝血研究实验室的信任。

Human α-Thrombin

 

HT 1002a              Human α-Thrombin (Factor IIa)

Prepared from homogeneous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. Human Thrombin purity is determined by SDS-PAGE. This activated enzyme has a minimum activity of 2,700 NIH units/mg when compared to NIH standard thrombin.

Buffer composition = 50 mM Sodium Citrate/0.2 M NaCl/0.1% PEG-8000/pH 6.5
*Extinction Coefficient (1%) = 18.3 
Molecular Weight = 37,000 daltons


多肽定制Thrombin Receptor Peptide Ligand 编码

上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。

名称 Thrombin Receptor Peptide Ligand
编码
别名 Thrombin Receptor Peptide Ligand
纯度 80%,90%,95%,98%,99%
重量 1mg,5mg,10mg,50mg,100mg,1g
序列(单字母缩写) AFLARAA
序列(三字母缩写) H-Ala-Phe-Leu-Ala-Arg-Ala-Ala-OH (trifluoroacetate salt)
基本描述
溶解度
分子量 718.9
化学式
存储条件 Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents Thrombin Receptor Peptide Ligand          编码
Figures Thrombin Receptor Peptide Ligand          编码
Reference Pakala, R. et al. Thromb Res. 100, 89 (2000) Pakala, R. et al. J. of Cardiovascular Pharm. 37, 619 (2001)
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多肽定制Thrombin sensitive peptide 编码

上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。

名称 Thrombin sensitive peptide
编码
别名 Thrombin sensitive peptide
纯度 80%,90%,95%,98%,99%
重量 1mg,5mg,10mg,50mg,100mg,1g
序列(单字母缩写) azidoacetyl alanine-K(5FAM)GALVPRGSAGK(CPQ2)
序列(三字母缩写) azidoacetyl alanine-Lys-(5FAM)-Gly-Ala-Leu-Val-Pro-Arg-Gly-Ser-Ala-Gly-Lys(CPQ2)-OH (trifluoroacetate salt)
基本描述 This peptide is the functional factor XIIIa substrate. Alpha-2-plasmin inhibitor, also termed primary plasmin inhibitor or antiplasmin, is the most potent and rapidly acting of the plasmin inhibitors and is thought to be important in the regulation of fibrinolysis in vivo. It has been used in assays to develop the potential of fibrin to promote nerve regeneration by enzymatically incorporating exogenous neurite-promoting heparin-binding peptides.
溶解度
分子量 2143
化学式
存储条件 Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents Thrombin sensitive peptide          编码
Figures Thrombin sensitive peptide          编码
Reference
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多肽定制Thrombin substrate 编码

上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。

名称 Thrombin substrate
编码
别名 Thrombin substrate
纯度 80%,90%,95%,98%,99%
重量 1mg,5mg,10mg,50mg,100mg,1g
序列(单字母缩写) 5FAM-GGfPRSGGGK(CPQ2)-K-PEG2-C
序列(三字母缩写) 5FAM-Gly-Gly-DPhe-Pro-Arg-Ser-Gly-Gly-Gly-Lys(CPQ2)-Lys-PEG2-Cys-OH (trifluoroacetate salt)
基本描述 This peptide, derived from the latency-associated peptide, inhibits thrombospondin (TSP-1) activation of TGF-beta; thus preventing the progression of hepatic damage and fibrosis.
溶解度
分子量 0
化学式
存储条件 Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents Thrombin substrate          编码
Figures Thrombin substrate          编码
Reference
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多肽定制Thrombin Substrate III, Fluorogenic 编码

上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。

名称 Thrombin Substrate III, Fluorogenic
编码
别名 Thrombin Substrate III, Fluorogenic
纯度 80%,90%,95%,98%,99%
重量 1mg,5mg,10mg,50mg,100mg,1g
序列(单字母缩写) Benzoyl-FVR-AMC
序列(三字母缩写) Benzoyl-Phe-Val-Arg-AMC (trifluoroacetate salt)
基本描述 A stromelysin-1 substrate, which is also hydrolyzed by two other matrix metalloproteinases, collagenase and gelatinase, and by the bacterial metalloproteinase thermolysin. The thiopeptolide Ac-PLA-psi[COS]Nva-W-amide mimics the MMP-3 cleavage site Leu-Ala-Nva-Trp
溶解度
分子量 681.8
化学式 C37H43N7O6
存储条件 Store at -20°C. Keep tightly closed. Store in a cool dry place.
注释
Documents Thrombin Substrate III, Fluorogenic          编码
Figures Thrombin Substrate III, Fluorogenic          编码
Reference L. Svendsen, et al., Folia Haematol, 98, 446 (1972) R. Lottenberg, et al., Meth. Enz., 80, 341 (1981)
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