pLVX-DsRed-Monomer-N1载体


 pLVX-DsRed-Monomer-N1载体

型号 载体名称 出品公司 载体用途
VMC0379 pLVX-DsRed-Monomer-N1 Clontech 慢病毒载体

Description

pLVX-DsRed-Monomer-N1 is an HIV-1-based, lentiviral expression vector that allows you 

to express your gene of interest fused to DsRed-Monomer, a monomeric mutant of the 

Discosoma sp. red fluorescent protein. Genes cloned into the multiple cloning site (MCS), 

located upstream of the DsRed-Monomer coding sequence, are expressed as N-terminal 

fusions of the DsRed-Monomer protein. Expression of the fusion protein is driven by the 

constitutively active human cytomegalovirus immediate early promoter (P

CMV IE) located 

just upstream of the MCS. Lentiviral particles derived from the vector allow the expression 

of DsRed-Monomer fusion proteins in virtually any cell type, including primary cells. The 

unmodified vector expresses DsRed-Monomer, and may be used to produce marker virus to 

optimize infection protocols.

质粒图谱: 

RNAi-Ready pSIREN-Retro Q-DsRed-Express

RNAi载体,RNAi-Ready pSIREN-Retro Q-DsRed-Express载体 

RNAi-Ready pSIREN-Retro Q-DsRed-Express

型号 载体名称 出品公司 载体用途
VRC0346 RNAi-Ready pSIREN-Retro Q-DsRed-Express Clontech RNAi载体
载体描述: 

RNAi-Ready pSIREN-RetroQ-DsRed-Express is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter (PU6; RNA Pol III-dependent). RNAi-Ready pSIREN-RetroQ-DsRed-Express is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when a ds DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQDsRed-Express construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA.

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