Cell Meter BX650固定化细胞活性染料
 |
货号 |
22520 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
200 Tests |
价格 |
2472 |
| Ex (nm) |
518 |
Em (nm) |
654 |
| 分子量 |
844.83 |
溶剂 |
DMSO |
| 产品详细介绍 |
简要概述
产品基本信息
货号：22520
产品名称：Cell Meter BX650固定化细胞活性染料
规格：200 Tests
储存条件：保存在冰箱-15℃干燥
保质期：12个月
产品物理化学光谱特性
分子量：N/A
激发波长(nm)：518
发射波长(nm)：654
适用仪器
| 流式细胞仪 |
|
| 激发： |
480nm激光 |
| 发射： |
695/40nm滤波片 |
| 滤波片： |
PerCP滤波片组 |
| 荧光显微镜 |
|
| 激发： |
TexasRed滤波片组 |
| 发射： |
TexasRed滤波片组 |
| 推荐孔板： |
黑色透明 |
产品介绍
从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter BX650固定化细胞活性染料是一类细胞不可渗透的荧光活性染料，它们经过优化以匹配常见流式细胞仪的主要激发激光，例如350、405、488、633和647 nm。这些染料不渗入活细胞，但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter BX650固定化细胞活性染料发生反应，因此比具有完整膜的活细胞的染色更亮，因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时，需要考虑一些因素，例如，每种染料的滴定，以确保活细胞几乎没有染色。Cell Meter BX650固定化细胞活性染料经过优化，可以在488 nm的蓝色激光激发下以650 nm的波长发射。与其他商业上类似的活性染料相比，这种固定化细胞活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Meter BX650固定化细胞活性染料。
点击查看光谱
图示
图1.通过Cell Meter 固定化细胞活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter BX650（Cat＃22520）染色，然后固定在3.7％甲醛中，并通过流式细胞仪进行分析。带有PerCP通道的死细胞群体（蓝色峰）很容易与活细胞群体（红色峰）区分开，在固定之前和之后都获得了几乎相同的结果。
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参考文献
Fluorescence-based method is more accurate than counting-based methods for plotting growth curves of adherent cells.
Authors: Pereira, Túlio Felipe and Levin, Gabriel and DeOcesano-Pereira, Carlos and Caodaglio, Amanda Schiersner and Fujita, André and Tonso, Aldo and Sogayar, Mari Cleide
Journal: BMC research notes (2020): 57
[Immune function of myeloid-derived suppressor cells and its mechanism in obstructive sleep apnea syndrome].
Authors: Chen, S W and Li, J and Xiang, B and Xu, S J and Wang, L
Journal: Zhonghua yi xue za zhi (2020): 295-300
CFDA-SE Combined with MACSiBeads™ Particles to Evaluate the Inhibitory Effect of Treg Cells in vitro.
Authors: Ren, Qingqi and Jiang, Chunlin and Liu, Jikui
Journal: Annals of clinical and laboratory science (2019): 740-747
Tracking keratinocytes and melanocytes using carboxyfluorescein hydroxysuccinimidyl ester staining.
Authors: Lönnqvist, Susanna and Junker, Johan P E and Sedell, Maria and Nyman, Erika and Kratz, Gunnar
Journal: PloS one (2019): e0221878
[Effects of skin γδ T lymphocytes on wound healing of mice through regulating proliferation and differentiation of mice epidermal cells].
Authors: Zhu, H J and Li, Y S and Wang, Y P and Hu, X H and Zhang, X R and Qiu, L and He, W F and Luo, G X
Journal: Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns (2019): 298-307
Impaired Immunosuppressive Effect of Bronchoalveolar Mesenchymal Stem Cells in Hypersensitivity Pneumonitis: Preliminary Findings.
Authors: Balogh, Enikő and Nagy, Béla and Gyetvai, Ágnes and Bene, Zsolt and Hendrik, Zoltán and Jeney, Viktória and Nagy, Péter and Papp, Ágnes and Balla, József and Balla, György and Kappelmayer, János and Nagy, Béla
Journal: Cytometry. Part B, Clinical cytometry (2018): 363-368
Delineating the distinct role of AKT in mediating cell survival and proliferation induced by CD154 and IL-4/IL-21 in chronic lymphocytic leukemia.
Authors: Chapman, Elinor A and Oates, Melanie and Mohammad, Ishaque S and Davies, Barry R and Stockman, Paul K and Zhuang, Jianguo and Pettitt, Andrew R
Journal: Oncotarget (2017): 102948-102964
Human auricular chondrocytes with high proliferation rate show high production of cartilage matrix.
Authors: Ishibashi, Makiko and Hikita, Atsuhiko and Fujihara, Yuko and Takato, Tsuyoshi and Hoshi, Kazuto
Journal: Regenerative therapy (2017): 21-28
[Tumor derived IgG suppress the proliferation of T cells in cord blood].
Authors: Liu, E Y and Liu, J F and Shao, W W and Xiao, L and Li, G H and Chang, X H and Qiu, X Y
Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2017): 824-828
A comparative study of colorimetric cell proliferation assays in immune cells.
Authors: Koyanagi, Madoka and Kawakabe, So and Arimura, Yutaka
Journal: Cytotechnology (2016): 1489-98
说明书
Cell Meter BX650固定化细胞活性染料.pdf
