样品实验方案
简要概述
- 在组织切片上涂抹即用型Stayright Purple溶液。
- 孵育组织切片5-15分钟。
- 漂洗组织5-10分钟，复染。
- 添加安装介质以覆盖该部分。
实验步骤
1.涂抹即用的Stayright Purple溶液。在室温下孵育5-15分钟。
2.将载玻片浸入dH2O中以停止颜色显影并监控染色强度。 如果染色强度不够明亮，则需要更长的孵育时间。 您可以重新应用Stayright Purple溶液来继续开发。
3.用dH2O洗涤5-10分钟。
4.如果需要，请使用所需的复染色。
5.用乙醇脱水，然后永久固定在有机永久固定介质中。
图示
图1. FFPE肺腺癌组织中EpCAM的免疫组织化学检测。 将组织切片与多HRP共轭的山羊抗兔IgG孵育，然后分别用Stayright Purple（左）和DAB（右）显影。 细胞用苏木精复染。 Stayright Purple产生的深层污渍具有高灵敏度和清晰的分辨率，类似于DAB。
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图2.固定了人肺腺癌组织样品并对其进行了EpCAM染色。 将组织切片与抗EpCAM兔单克隆抗体（左）或同种型IgG作为阴性对照（右）一起孵育。 然后将样品与结合了HRP的山羊抗兔IgG抗体孵育，并用Stayright Purple溶液显影信号。 Stayright Purple可产生干净的紫色荧光，无背景。
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