Abraxis Legionella 说明书

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Legionella

Legionella bacteria, the cause of Legionnaire’s disease (a deadly pneumonia), aren’t just an historical problem—cases are on the rise in the US and around the world.

From Science News, Sept. 7, 2015: This summer, in New York City’s worst ever outbreak of Legionnaires’ disease, left 12 dead and at least 100 infected. In two different outbreaks in an Illinois town in recent days at least 50 people have been diagnosed with the disease and 8 reported dead. Also in a California prison, 95 inmates are being watched, being suspected of being infected and six inmates were already confirmed to have contracted the disease.

The Centers for Disease Control (CDC), Occupational Safety & Health Administration (OSHA), and World Health Organization (WHO) have guidelines for detection, prevention, remediation, and treatment of Legionella. The release of ANSI/ASHRAE Standard 188-2015 and VHA Directive 1061 provide guidance for establishing a Water Management Program.

Abraxis is pleased to distribute Legipid® from Biotica, the only Legionella rapid detection system that quantitatively measures viable Legionella in water samples (from cooling towers, evaporative condensers, spas, pools, showers, sprinkler systems, surface water, etc.). It is an Immunomagnetic Separation Capture Enzyme Immunoassay (IMS-CEIA) format that is easy to use and AOAC certified. Legipid is a screening kit for fast, accurate, quantitative results that allow rapid action while awaiting confirmation by the much slower standardized culture methods (e.g. ISO 11731:1998). For more information, 

Legipid Advantages:

● Fast: Up to 40 tests in 1 hour (after filtering)
● Selective for most (90%) Legionella spp. (unlike dipstick tests), including L. pneumophila
● Sensitive, equal to or better than culturing
● Visual or Optical (429 nm) Colorimetric Detection
● Quantitative, results easily converted to cfu
● Detects VBNC (Viable But Not Culturable) Legionella and biocide-treated samples
● Easy to Multiplex with other water test analyses
● Simple Workflow
● High Value: unlike other methods, no complicated/expensive equipment necessary
● Few False Negatives (unlike culture and PCR)
● Few False Positives (unlike PCR, which also detects dead cells and free DNA)
 

Legipid Workflow:
● Filter 1 liter water sample
● Add Legipid magnetic beads
● Wash and add antibody
● Add detection reagent
● Visual or Optical (429 nm) colorimetric detection

Legionella Products

Legipid® Legionella IMS-CEIA Kits 
Provides reagents and vials to perform Legipid tests; magnetic holders available separay (see below). Fitration/concentration systems available separay (see below).
PN 311-10-01 (10 tests) 
PN 311-10-02 (40 tests) 
PN 311-10-04 (100 tests) 

Legipid Magnetic Holders
MP Hunter 4
PN 311-MP4-SP Magnetic holder assembly, holds 4 test vials
PN 311-MP4-RA-M Holds 20 test vials (5 holder assemblies, 1 tablecloth, 1 platform & cover included)

Filtration/Concentration Systems
Filterpid Fast Filtration Kit
Easy, complete system (using 60 ml syringe) to prepare Legionella water samples for Legipid or Cleanpid.
PN 211-10-00 
Manual pump filtration system
PN 311-10-SFM-FP
Electric pump filtration system  
PN 311-10-SFC-FP 

Cleanpid® Legionella Easy Purification Kit for PCR  
Simple and rapid method for the separation and purification of Legionella spp. in potable, natural, and industrial water. The method is based on magnetic bead immunocapture of Legionella cells after pre-concentration of samples (see filtration/concentration systems above).
PN 611-10-00 (50 tests)
PN 611-MRC-10 Magnetic holder for 10 tubes

Other Products
Primelab Photometer  
Absorbance/optical reader (380 nm – 780 nm) suitable for Legipid and other water tests, includes cuvette adaptor and 100 microcuvettes.
PN 911-10-PL
AllinOne portable lab-in-a-case  
Portable lab-in-a-case for on-site Legionella detection; includes magnetic holder, electric pump filtration system, and Primelab photometer. Purchase Legipid reagents separay (see kits above).
PN 411-10-AIO
Orbital Shaker
PN 706000 For use with Legipid MP Hunter 4 Magnetic holder assemblies
Vial, Round, Glass
For use with Legipid kit
PN 311-10-CB (quantity 1)