Ethidium Homodimer-I (EthD-I) 货号: BN14052

Ethidium Homodimer-I (EthD-I)
Ethidium Homodimer-I (EthD-I)       货号: BN14052Ethidium Homodimer-I (EthD-I)       货号: BN14052Ethidium Homodimer-I (EthD-I)       货号: BN14052

  • 产品货号:
    BN14052
  • 中文名称:
    Ethidium Homodimer-I (EthD-I)
  • 英文名称:
    Ethidium Homodimer-I (EthD-I)
  • 品牌:
    Biorigin

  • 货号

    产品规格

    售价

    备注

  • BN14052-1 mg

    1 mg

    ¥1560.00

    染色剂/指示剂

产品描述

应用范围:核酸染色

贮存条件: 4℃避光保存

产品参数 

外观:可溶于 DMSO 和 MeOH 的红色固体 

λEx/λEm = 528/617 nm(结合 DNA) 

λEx/λEm = 493 nm(未结合 DNA) 

保质期:12 个月 

CAS 号:61926-22-5 

分子式:C46H50Cl4N8 

分子量:857 

分子结构图:

Ethidium Homodimer-I (EthD-I)       货号: BN14052

产品介绍 

Ethidium Homodimer-I (EthD-I)是一种高亲和性的荧光核酸染料,与 DNA 或 RNA 结合后,可以使荧光增强 30 多倍。用于哺乳动物、细菌、酵母和真菌的染色。Ethidium Homodimer-I (EthD-I)带有较强正电荷,所以该染料不能穿过细胞膜进行活细胞染色。但是 EthD-I 可以准确的检测溶液中的核酸或者解体细胞中的核酸,是一种较灵敏的核酸染色剂。

使用方法 

注:该操作说明适用于大多数细胞,但不同的细胞类型、细胞密度、使用的培养基以及其他一些因素都有可能影响染色效果,本说明仅供参考。

1. 储存液的制备:取适量DMSO加入到Ethidium Homodimer-I中,配制成2mM储液,该储存液可于-20℃稳定保存一年。

2. 将20μl 2mM储液加入到10ml无菌的组织培养级别的 D-PBS中,充分涡旋混匀,使其终浓度为4μM(推荐浓度为 0.1-10μM,不同细胞系建议梯度设置确定最佳染色浓度)。

3. 吸取上述配置好的工作液100-150μl加入到细胞盖玻片上使其完全覆盖。孵育最好是在含有盖子的盘子里防止染色液挥发。

4. 室温孵育30-45min,若染色液浓度过高或温度过低可适当减少孵育时间。 

5. 向一个新的显微镜载玻片上加入10μl D-PBS。 

6. 使用尖镊子小心且迅速将载有细胞的盖玻片倒置加在含有D-PBS的载玻片上,为了防止染色液挥发,用干净透明的指甲油封住载玻片四周。 

7. 在荧光显微镜下观察细胞染色情况。

注意事项 

1. 荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。 

2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

  • Ethidium Homodimer-I (EthD-I)       货号: BN14052 说明书下载.pdf

死细胞核染料EthD-1 [Ethidium Homodimer-1](停产) CAS 61926-22-5 货号17780-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

死细胞核染料EthD-1 [Ethidium Homodimer-1](停产) CAS 61926-22-5

死细胞核染料EthD-1 [Ethidium Homodimer-1](停产) CAS 61926-22-5

死细胞核染料EthD-1 [Ethidium Homodimer-1](停产) CAS 61926-22-5    货号17780 货号 17780 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 0
Ex (nm) Em (nm)
分子量 856.76 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:17780

产品名称:死细胞核染料EthD-1 [Ethidium Homodimer-1]

CAS:61926-22-5

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:856.76

外观:固体

溶剂:DMSO

 

产品介绍

Ethidium Homodimer-1 (EthD-1) 是一种高亲和力荧光核酸染料,可结合 ssDNA、dsDNA、RNA、寡核苷酸和三螺旋 DNA。 与DNA结合后,其荧光增强(>30倍),并发出鲜红色荧光。 由于 EthD-1 带有强正电荷,不能穿透完整/活细胞膜,因此通常用于检测死细胞。 EthD-1 通常与 Calcein AM 结合使用,分别对死细胞和活细胞进行染色,以确定细胞活力和毒性。 EthD-1 是一种适用于所有细胞类型(包括哺乳动物、细菌和酵母)的死细胞特异性染料。 它还可以与我们的其他细胞活力染料(例如优质的 Calcein Ultra Blue、Calcein Ultra Green、Cytocalcein、Cytotrace 染料和 CytoTell 染料)结合用于检测活/死细胞。 金畔生物是AAT Bioquest的中国代理商,为您提供最优质的细胞核染料。 

 

参考文献

Designing and validation of an automated ex-vivo bioreactor system for long term culture of bone.
Authors: Dua, Rupak and Jones, Hugh and Noble, Philip C
Journal: Bone reports (2021): 101074

Designing of spider silk proteins for human induced pluripotent stem cell-based cardiac tissue engineering.
Authors: Esser, T U and Trossmann, V T and Lentz, S and Engel, F B and Scheibel, T
Journal: Materials today. Bio (2021): 100114

The Silk Protein Sericin Promotes Viability of ARPE-19 and Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells in vitro.
Authors: Khan, Ayyad Zartasht and Utheim, Tor Paaske and Moe, Morten Carstens and Aass, Hans Christian D and Sapkota, Dipak and Vallenari, Evan Michael and Eidet, Jon Roger
Journal: Current eye research (2021): 504-514

Butaphosphan and Cyanocobalamin Supplementation in Semen Extender on Chilled Boar Sperm Quality and Life Span.
Authors: Suwimonteerabutr, J and Chumsri, S and Tummaruk, P and Nuntapaitoon, Morakot
Journal: Frontiers in veterinary science (2020): 592162

Impacts of different synthetic polymers on vitrification of ovarian tissue.
Authors: Shahsavari, Mohammad Hamed and Alves, Kele Amaral and Alves, Benner Geraldo and de Lima, Laritza Ferreira and Vizcarra, Diego Alberto Montano and Berrocal, Deysi Juana Dipaz and Silva, Luciana Mascena and da Silva, Yago Pinto and Zelinski, Mary B and de Figueiredo, José Ricardo and Moghaddam, Gholamali and Rodrigues, Ana Paula Ribeiro
Journal: Cryobiology (2020): 66-72

Influence of Tooth Pigmentation on H2O2 Diffusion and Its Cytotoxicity After In-office Tooth Bleaching.
Authors: de Oliveira Duque, C C and Soares, D G and Briso, Alf and Ortecho-Zuta, U and de Oliveira Ribeiro, R A and Hebling, J and de Souza Costa, C A
Journal: Operative dentistry (2020): 632-642

Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model.
Authors: Mamani, Javier Bustamante and Marinho, Bruna Souto and Rego, Gabriel Nery de Albuquerque and Nucci, Mariana Penteado and Alvieri, Fernando and Santos, Ricardo Silva Dos and Ferreira, João Victor Matias and Oliveira, Fernando Anselmo de and Gamarra, Lionel Fernel
Journal: Einstein (Sao Paulo, Brazil) (2020): eAO4954

Optisol-GS Storage of Cultured Human Limbal Epithelial Cells at Ambient Temperature Is Superior to Hypothermic Storage.
Authors: Jackson, Catherine Joan and Pasovic, Lara and Raeder, Sten and Sehic, Amer and Roald, Borghild and de la Paz, Maria F and Tønseth, Kim Alexsander and Utheim, Tor Paaske
Journal: Current eye research (2020): 1497-1503

Povidone iodine treatment is deleterious to human ocular surface conjunctival cells in culture.
Authors: Swift, William and Bair, Jeffrey A and Chen, Wanxue and Li, Michael and Lie, Sole and Li, Dayu and Yang, Menglu and Shatos, Marie A and Hodges, Robin R and Kolko, Miriam and Utheim, Tor P and Scott, Wendell and Dartt, Darlene A
Journal: BMJ open ophthalmology (2020): e000545

Preclinical ex-vivo Testing of Anti-inflammatory Drugs in a Bovine Intervertebral Degenerative Disc Model.
Authors: Li, Zhen and Gehlen, Yannik and Heizmann, Fabian and Grad, Sibylle and Alini, Mauro and Richards, R Geoff and Kubosch, David and Südkamp, Norbert and Izadpanah, Kaywan and Kubosch, Eva Johanna and Lang, Gernot
Journal: Frontiers in bioengineering and biotechnology (2020): 583

说明书
死细胞核染料EthD-1 [Ethidium Homodimer-1](停产) CAS 61926-22-5.pdf

死细胞核染料EthD-2 [Ethidium Homodimer-2](停产) 货号17782-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

死细胞核染料EthD-2 [Ethidium Homodimer-2](停产)

死细胞核染料EthD-2 [Ethidium Homodimer-2](停产)

死细胞核染料EthD-2 [Ethidium Homodimer-2](停产)    货号17782 货号 17782 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 0
Ex (nm) Em (nm)
分子量 1292.71 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:17782

产品名称:死细胞核染料EthD-2 [Ethidium Homodimer-2]

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1292.71

外观:固体

溶剂:DMSO

 

产品介绍

Ethidium Homodimer-2 (EthD-2) 通常用作细胞非渗透性活力指标。 它是一种高亲和力核酸染色剂,在与 DNA 结合之前呈微弱荧光,并发出红色荧光(激发/发射最大值 ~535/624)。 它通常与钙黄绿素 AM 一起使用。 通过用钙黄绿素乙酰氧基甲酯 (Calcein AM) 和 EthD-2 染色,可以很容易地评估细胞的活力。 活细胞(绿色,用 Calcein AM 染色)和死细胞(红色,用 EthD-2 染色)可以很容易地成像,甚至可以使用 ImageJ 程序从图像中量化。 它还可以与我们的其他细胞活力染料结合使用,例如卓越的 Calcein Ultra Blue、Calcein Ultra Green、Cytocalcein、Cytotrace 染料和 CytoTell 染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的细胞核染料。 

 

参考文献

Iatrogenic Articular Cartilage Injury in Arthroscopic Hip and Knee Videos and the Potential for Cartilage Cell Death When Simulated in a Bovine Model.
Authors: Compton, Jocelyn and Slattery, Michael and Coleman, Mitchell and Westermann, Robert
Journal: Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association (2020): 2114-2121

In Vitro Effect of Vancomycin on the Viability of Articular Chondrocytes.
Authors: Shaw, K Aaron and Eichinger, Josef K and Nadig, Nischal and Parada, Stephen A
Journal: Journal of orthopaedic trauma (2018): 148-153

Chondrocyte Viability After a Simulated Blast Exposure.
Authors: Shaw, K Aaron and Johnson, Peter C and Williams, David and Zumbrun, Steven D and Topolski, Richard and Cameron, Craig D
Journal: Military medicine (2017): e1941-e1947

Why Do Osteochondral Allografts Survive? Comparative Analysis of Cartilage Biochemical Properties Unveils a Molecular Basis for Durability.
Authors: Ding, Lei and Zampogna, Biagio and Vasta, Sebastiano and Jang, Kee Woong and De Caro, Francesca and Martin, James A and Amendola, Annunziato
Journal: The American journal of sports medicine (2015): 2459-68

DNA counterstaining for methylation and hydroxymethylation immunostaining in bovine zygotes.
Authors: Heras, Sonia and Forier, Katrien and Rombouts, Koen and Braeckmans, Kevin and Van Soom, Ann
Journal: Analytical biochemistry (2014): 14-6

Pretreatment of therapeutic cells with poly(ADP-ribose) polymerase inhibitor enhances their efficacy in an in vitro model of cell-based therapy in myocardial infarct.
Authors: Szepes, Mónika and Janicsek, Zsófia and Benkő, Zsolt and Cselenyák, Attila and Kiss, Levente
Journal: International journal of molecular medicine (2013): 26-32

Reliability of Hoechst 33342 staining under wide-field microscopy for evaluation of the nuclear status of living dog oocytes.
Authors: Chebrout, Martine and Adenot, Pierre-Gaël and Reynaud, Karine and Chastant-Maillard, Sylvie
Journal: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada (2012): 483-92

Nuclear and cytoplasmic maturation of canine oocytes related to in vitro denudation.
Authors: Chebrout, M and de Lesegno, C Viaris and Reynaud, K and Chat, S and Chastant-Maillard, S
Journal: Reproduction in domestic animals = Zuchthygiene (2009): 243-6

C5 inhibitor rEV576 protects against neural injury in an in vitro mouse model of Miller Fisher syndrome.
Authors: Halstead, Susan K and Humphreys, Peter D and Zitman, Femke M P and Hamer, John and Plomp, Jaap J and Willison, Hugh J
Journal: Journal of the peripheral nervous system : JPNS (2008): 228-35

Continuous exposure to bisphenol A during in vitro follicular development induces meiotic abnormalities.
Authors: Lenie, Sandy and Cortvrindt, Rita and Eichenlaub-Ritter, Ursula and Smitz, Johan
Journal: Mutation research (2008): 71-81

说明书
死细胞核染料EthD-2 [Ethidium Homodimer-2](停产).pdf