NIGHTSEA荧光观测设备怎么用
美国NIGHTSEA专门致力于研究和开发荧光观测装备。NIGHTSEA转基因生物观测利器包括：SFA-RB体视显微镜荧光适配器，DFP-1双荧光蛋白手电筒系列，BLS2单荧光蛋白手电筒系列等。
（一）美国NIGHTSEA转基因生物观测利器的应用之——转基因生物观测：如小鼠幼崽，果蝇幼虫，线虫，斑马鱼，等等。
1. 美国NIGHTSEA转基因生物观测利器—观测转基因果蝇
2. 美国NIGHTSEA转基因生物观测利器—观测转基因斑马鱼
3. 美国NIGHTSEA转基因生物观测利器—观测转基因爪蟾
4. 美国NIGHTSEA转基因生物观测利器—观测转基因线虫
5.美国NIGHTSEA转基因生物观测利器—观测转基因蝾螈
6.美国NIGHTSEA转基因生物观测利器—观测转基因拟南芥种子
（二）美国NIGHTSEA转基因生物观测利器的应用之——样本预筛选和辅助解剖
荧光是细胞生物学、神经生物学等多个研究领域中广泛使用的工具。nightsea的DFP-1, BLS2和SFA-RB可以用于样本的预筛选、辅助解剖等，下面列举几个实例：
1. 荧光蛋白标记的工程病毒载体在神经元功能研究中应用十分广泛，常通过颅内注射研究特定脑区的功能。在此中，常需要对病毒表达区域进行快速解剖用于特定的生化分析。为了提高快速解剖定位度，研究人员选用nightsea的BLS2 Bluestar手电筒观测系列，通过配合nightsea滤光眼镜准确定位注射表达了荧光蛋白的伏核区(文献– Xuan Li and Marina E. Wolf, 2011. Visualization of virus-infected brain regions using a GFP-illuminating flashlight enables accurate and rapid dissection for biochemical analysis. J. of Neuroscience Methods, Vol. 201, Issue 1, pp. 177-179. )
EGFP标记的慢病毒载体注射进小鼠大脑区域（Marina Wolf, Rosalind Franklin University）
2. 研究者从小鼠大脑中提取GFP标记的背纹体。这项困难的工作因有了NIGHTSEA的DFP-1或BLS2（手电筒和眼镜组合）而变得简单：很容易看到大脑中的目标区域，让解剖更准确、。
小鼠大脑中GFP标记的背纹体（Charles Mazel. Sample photographed at laboratory of Stefano Vicini, Georgetown University）
3. 用DFP-1双荧光蛋白手电筒，BLS2单荧光蛋白手电筒，或SFA-RB体视显微镜荧光适配器，可以快速检测样本是否成功染色（Alexa Fluor 488 Phalloidin标记），如下图：
兔腰肌的肌纤维（用Alexa Fluor 488 Phalloidin标记）
左图：白光观测 右图：Royal Blue荧光观测（Motic SMZ-186体视显微镜观测）
4.用SFA-RB体视显微镜荧光适配器以斑马鱼为模型，观测不同有害化学物（医药、农药、食品添加剂等）对大脑发育的影响。观测图片如下：
基因斑马鱼大脑共聚焦图像
图片由Robert Thorn（Creton Lab, 布朗大学）提供
5. 荧光是珊瑚采集研究的重要工具。现在，可以用经济又实惠的SFA-RB体视显微镜荧光适配器安装到体视显微镜上检测珊瑚。
下面的图片是通过立体显微镜观看珊瑚虫，由UNCW威尔明顿纳大学Alina Szmant 博士与NIGHTSEA Charles Mazel博士提供
6. 用于结核分枝杆菌重组株的筛选
在结核分枝杆菌中成功构建了同源重组系统，利用该系统构建了rv1364c、pstP跨膜区、pstP胞外区三个突变株，得到双交换突变株的效率为25％-62.5％，从双交换突变株得到无痕缺失突变株的效率为100％。通过gfp作为荧光标记基因，利用NightSea BlueStar蓝光手电筒和滤光眼镜,可以对平板上的基因缺失株菌落直接进行快速判定。
采用nightsea BLS2 BlueStar 蓝色手电筒和滤光镜对平板上的结核分枝杆菌绿色荧光蛋白菌落进行快速鉴定。该方法可以从几百个菌落中瞬间锁定阳性克隆。平板上的菌落照片拍摄用单反相机加载购自nightsea公司的滤光片实现。
图片源自[结核分枝杆菌同源重组基因敲除系统的构建和应用]
荧光是细胞生物学、神经生物学等领域中广泛使用的工具。美国nightsea致力于研究和开发荧光观测装备，其产品系列有DFP-1双荧光蛋白手电筒系列，BLS2单荧光蛋白手电筒系列，SFA-RB体视显微镜荧光适配器等。
DFP-1 Dual Fluorescent Protein Flashlight 双荧光蛋白手电筒系列
DFP-1双荧光蛋白手电筒，有两个可转换的高强度LED，可激发高强度光源。目前，绿色荧光（GFP、EGFP、fluorescein等）和红色荧光（DsREed，TdTomato等）是常用的类型，nightsea常规的DFP-1双荧光蛋白手电筒是激发蓝光和绿光，再分别使用黄色滤光镜和红色滤光镜，就可以分别检测绿色荧光蛋白（GFP、EGFP、fluorescein等）和红色荧光蛋白（DsREed，TdTomato等）了。
Good News
标准的DFP-1双荧光蛋白观测镜，只有RB-Royal Blue和GR-Green两种荧光激发装置，也就是说标准的DFP-1只能用于观测绿色荧光蛋白和红色荧光蛋白。
现在，您可以选择以下任何两种荧光装置，组装成自己专属的双荧光蛋白手电筒。
|
荧光装置型号
|
Excitation
激发光
|
Emission
发射光
|
可观测荧光
|
|
RB-Royal Blue
|
440-460nm
|
500nm LP
|
GFP, eGFP, fluorescein….
|
|
CY-Cyan
|
490-515nm
|
550nm LP
|
YFP, Venus, Lucifer Yellow…
|
|
GR-Green
|
510-540nm
|
600nm LP
|
DsRed, dTomato…
|
|
VI-Violet
|
400-415nm
|
460nm LP
|
CFP, BFP…
|
|
UV-Ultra Violet
|
360-380nm
|
415nm LP
|
DAPI
|
注：
1.RB-Royal Blue用于观测绿色荧光蛋白
GFP：绿色荧光；EGFP：增强型绿色荧光；fluorescein荧光素
2.CY-Cyan用于观测黄色荧光蛋白
YFP：黄色荧光蛋白；Venus：金色；Lucifer Yellow：荧光黄
3.GR-Green用于观测红色荧光蛋白
DsRed：红色荧光蛋白；dTomato：番茄红
4.VI-Violet用于观测紫色荧光蛋白
CFP：青色荧光蛋白；BFP：碱性胎儿蛋白
5.UV-Ultra Violet用于观测紫外光
DAPI：蓝色荧光
nightsea 2018年 产品列表：
|
货号
|
品名
|
规格
|
价格
|
品牌
|
|
SFA-TENT
|
Eclipse MicroTent
|
each
|
5525
|
nightsea
|
|
SFA-EYE-S
|
Tru-Block Eye Shields – Standard
|
set
|
306
|
nightsea
|
|
SFA-EYE-C
|
Tru-Block Eye Shields – Compact
|
set
|
306
|
nightsea
|
|
SFA-BLH-RB
|
Light Head-Royal Blue
|
each
|
4250
|
nightsea
|
|
SFA-BLH-CY
|
Light Head-Cyan
|
each
|
4250
|
nightsea
|
|
SFA-BLH-GR
|
Light Head-Green
|
each
|
4250
|
nightsea
|
|
SFA-BLH-VI
|
Light Head-Violet
|
each
|
4250
|
nightsea
|
|
SFA-BLH-UV
|
Light Head-Ultraviolet
|
each
|
6545
|
nightsea
|
|
SFA-BF-RB
|
NIGHTSEA Barrier Filter-Royal Blue
|
each
|
3536
|
nightsea
|
|
SFA-BF-GO
|
NIGHTSEA Barrier Filter-Green Only
|
each
|
3536
|
nightsea
|
|
SFA-BF-CY
|
NIGHTSEA Barrier Filter-Cyan
|
each
|
3536
|
nightsea
|
|
SFA-BF-GR
|
NIGHTSEA Barrier Filter-Green
|
each
|
3536
|
nightsea
|
|
SFA-BF-VI
|
NIGHTSEA Barrier Filter-Violet
|
each
|
3536
|
nightsea
|
|
SFA-BF-UV
|
NIGHTSEA Barrier Filter-Ultraviolet
|
each
|
3536
|
nightsea
|
|
SFA-SH-RB
|
NIGHTSEA Shield-Royal Blue
|
each
|
1785
|
nightsea
|
|
SFA-SH-GO
|
NIGHTSEA Shield-Green Only
|
each
|
1785
|
nightsea
|
|
SFA-SH-CY
|
NIGHTSEA Shield-Cyan
|
each
|
1785
|
nightsea
|
|
SFA-SH-GR
|
NIGHTSEA Shield-Green
|
each
|
1785
|
nightsea
|
|
SFA-SH-VI
|
NIGHTSEA Shield-Violet
|
each
|
1785
|
nightsea
|
|
SFA-SH-UV
|
NIGHTSEA Shield-Ultraviolet
|
each
|
1785
|
nightsea
|
|
SFA-AD
|
NIGHTSEA Adapter
|
each
|
2856
|
nightsea
|
|
SFAZ-AD
|
Leica EZ4 Adapter only
|
each
|
2856
|
nightsea
|
|
SFA-XL-AD
|
NIGHTSEA Oversize Adapter
|
each
|
2856
|
nightsea
|
|
SFA-DIM-N
|
NIGHTSEA DIM Base
|
each
|
11305
|
nightsea
|
|
SFA-PULSE-N
|
NIGHTSEA PULSE Base
|
each
|
11305
|
nightsea
|
|
SFA-BASE
|
NIGHTSEA Standard Base
|
each
|
7055
|
nightsea
|
|
SFA-LHH-L
|
Light Head Hanger, long working distance
|
each
|
1615
|
nightsea
|
|
SFA-LHH-S
|
Light Head Hanger, short working distance
|
each
|
1615
|
nightsea
|
|
SFA-LHC
|
Single Light Head Cable, V1
|
each
|
510
|
nightsea
|
|
SFA-LHC-BNC
|
Single Light Head Cable, BNC
|
each
|
595
|
nightsea
|
|
SFA-DLHC
|
Dual Light Head Cable, V1
|
each
|
1105
|
nightsea
|
|
SFA-DLHC-BNC
|
Dual Light Head Cable, BNC
|
each
|
1105
|
nightsea
|
|
SFA-HK-L
|
Single hanger kit, long working distance, V1: SFA-LHC + SFA-LHH-L
|
kit
|
2125
|
nightsea
|
|
SFA-HK-S
|
Single hanger kit, short working distance, V1: SFA-LHC + SFA-LHH-S
|
kit
|
2125
|
nightsea
|
|
SFA-HK-L-BNC
|
Single hanger kit, long working distance, BNC: SFA-LHC-BNC + SFA-LHH-L
|
kit
|
2125
|
nightsea
|
|
SFA-HK-S-BNC
|
Single hanger kit, short working distance, BNC: SFA-LHC-BNC + SFA-LHH-S
|
kit
|
2125
|
nightsea
|
|
SFA-DHK-L
|
Dual hanger kit, long working distance, V1: SFA-DLHC + 2x SFA-LHH-L
|
kit
|
4165
|
nightsea
|
|
SFA-DHK-S
|
Dual hanger kit, short working distance, V1: SFA-DLHC + 2x SFA-LHH-S
|
kit
|
4165
|
nightsea
|
|
SFA-DHK-L-BNC
|
Dual hanger kit, long working distance, BNC: SFA-DLHC-BNC + 2x SFA-LHH-L
|
kit
|
4165
|
nightsea
|
|
SFA-DHK-S-BNC
|
Dual hanger kit, short working distance, BNC: SFA-DLHC-BNC + 2x SFA-LHH-S
|
kit
|
4165
|
nightsea
|
|
SFA-SWK-S-BNC
|
Switch Box Kit, short working distance: BNC switch box, 3 BNC cables, one with female/female gooseneck adapter, 2 LH Hangers
|
kit
|
5950
|
nightsea
|
|
SFA-SWK-L-BNC
|
Switch Box Kit, long working distance: BNC switch box, 3 BNC cables, one with female/female gooseneck adapter, 2 LH Hangers
|
kit
|
5950
|
nightsea
|
|
DFP-1
|
DFP Flashlight with RB and GR LEDs, matching filter glasses, carrying case
|
each
|
12835
|
nightsea
|
|
DFP-CG
|
DFP Flashlight with CY and GR LEDs, matching filter glasses, carrying case
|
each
|
13855
|
nightsea
|
|
DFP-RC
|
DFP Flashlight with RB and CY LEDs, matching filter glasses, carrying case
|
each
|
13855
|
nightsea
|
|
DFP-VC
|
DFP Flashlight with VI and CY LEDs, matching filter glasses, carrying case
|
each
|
13855
|
nightsea
|
|
DFP-VG
|
DFP Flashlight with VI and GR LEDs, matching filter glasses, carrying case
|
each
|
13855
|
nightsea
|
|
DFP-VR
|
DFP Flashlight with VI and RB LEDs, matching filter glasses, carrying case
|
each
|
13855
|
nightsea
|
|
DFP-UC
|
DFP Flashlight with UV and CY LEDs, matching filter glasses, carrying case
|
each
|
16065
|
nightsea
|
|
DFP-UG
|
DFP Flashlight with UV and GR LEDs, matching filter glasses, carrying case
|
each
|
16065
|
nightsea
|
|
DFP-UR
|
DFP Flashlight with UV and RB LEDs, matching filter glasses, carrying case
|
each
|
16065
|
nightsea
|
|
DFP-UV
|
DFP Flashlight with UV and VI LEDs, matching filter glasses, carrying case
|
each
|
16065
|
nightsea
|
|
BLS-1
|
BlueStar light plus style VG1 filter glasses
|
each
|
3485
|
nightsea
|
|
BLS-2
|
BlueStar light plus style VG2 filter glasses
|
each
|
3655
|
nightsea
|
|
BLS-3
|
BlueStar light plus style VG3 filter glasses
|
each
|
3655
|
nightsea
|
|
VG-1
|
Yellow filter glasses, style 1
|
each
|
578
|
nightsea
|
|
VG-2
|
Yellow filter glasses, style 2
|
each
|
935
|
nightsea
|
|
VG-3
|
Yellow filter glasses, style 3
|
each
|
935
|
nightsea
|
|
RG-2
|
Red filter glasses, style 2
|
each
|
935
|
nightsea
|
|
FG-UV
|
Filter glasses, Ultraviolet
|
each
|
935
|
nightsea
|
|
FG-VI
|
Filter glasses, Violet
|
each
|
935
|
nightsea
|
|
FG-GO
|
Filter glasses, Green Only
|
each
|
935
|
nightsea
|
|
FG-CY
|
Filter glasses, Cyan
|
each
|
935
|
nightsea
|
|
SFA-PULSE-R
|
PULSE Option – Retrofit
|
each
|
4675
|
nightsea
|
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