ReadiUse™Tyramide（TSA）/ Styramide（PSA）优化的反应缓冲液
| 货号 |
45090 |
存储条件 |
在2-8度冷藏保存 |
| 规格 |
1000 Reactions |
价格 |
1200 |
| Ex (nm) |
|
Em (nm) |
|
| 分子量 |
|
溶剂 |
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| 产品详细介绍 |
简要概述
对于许多免疫组织化学（IHC）应用，传统的酶促扩增程序足以实现足够的抗原检测。但是，有几个因素限制了这些程序的敏感性和实用性。酪酰胺信号放大（TSA）已被证明是一种特别通用且功能强大的酶放大技术，具有更高的测定灵敏度。TSA基于HRP在低浓度过氧化氢存在下将标记的含酪胺底物转化为可与HRP或附近的酪氨酸残基共价结合的氧化的高反应性自由基的能力。Power Styramide™信号放大（PSA™）是对TSA系统的最新升级，具有比相应TSA试剂高10至50倍的改进荧光信号。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TSA/PSA相关产品。
产品说明书
样品分析方案
概述
细胞或组织培养
在封闭缓冲液中添加一抗
加入结合HRP的二抗
准备Styramide™（PSA）或Tyramide（TSA）工作溶液，并在室温下在细胞或组织中涂抹5-10分钟
储备溶液配制
1.PSA储备溶液（100X）
将100μLDMSO加入到iFluor™染料标记的Styramide™缀合物（例如＃45000）的小瓶中，制成100X Styramide™储备液。
注意：制成单次使用的等分试样，并将未使用的100X储备溶液在2-8°C的暗处存储，并避免重复冻融循环。
2. H2O2储备溶液
向90μL的ddH 2 O中添加10μL的3％过氧化氢（组分B）。
注意：在使用当天准备新鲜的100X H 2 O 2溶液。
工作溶液配制
PSA工作溶液（1X）
每1 mL PSA工作溶液需要1 mL TSA / PSA缓冲液（组分A），10μLStyramide™储备溶液和10μLH 2 O 2储备溶液。
注意：1 mL足够进行10次测试，基于每个盖玻片或96孔微孔板中每孔所需的100μLStyramide™工作溶液。
注意：Styramide™工作溶液必须在制备后2小时内使用，并避免直接暴露在光线下。
参考文献
A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum.
Authors: Buescher, Marita and Oberhofer, Georg and Garcia-Perez, Natalia Carolina and Bucher, Gregor
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 219-232
Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping.
Authors: Roberts, Kenny and Bayraktar, Omer Ali
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 229-244
Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.
Authors: Wang, Herui and Pangilinan, Ryan L and Zhu, Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 89-97
Estimating the ploidy of Gracilariopsis lemaneiformis at both the cellular and genomic level.
Authors: Chen, Haihong and Feng, Xiaoqing and Jiang, Minjie and Xiao, Baoheng and Zhang, Jingyu and Zhang, Wei and Hu, Yiyi and Sui, Zhenghong
Journal: Journal of phycology (2020)
Liver fibrosis and CD206+ macrophage accumulation are suppressed by anti-GM-CSF therapy.
Authors: Tan-Garcia, Alfonso and Lai, Fritz and Sheng Yeong, Joe Poh and Irac, Sergio E and Ng, Pei Y and Msallam, Rasha and Tatt Lim, Jeffrey Chun and Wai, Lu-En and Tham, Christine Y L and Choo, Su P and Lim, Tony and Young, Dan Y and D’Ambrosio, Roberta and Degasperi, Elisabetta and Perbellini, Riccardo and Newell, Evan and Le Bert, Nina and Ginhoux, Florent and Bertoletti, Antonio and Chen, Qingfeng and Dutertre, Charles-Antoine
Journal: JHEP reports : innovation in hepatology (2020): 100062
Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies.
Authors: Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio
Journal: Cancers (2020)
Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish.
Authors: Lauter, Gilbert and Söll, Iris and Hauptmann, Giselbert
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 397-409
Tagging the vanA gene in wastewater microbial communities for cell sorting and taxonomy of vanA carrying cells.
Authors: Gallego, Sara and Barkay, Tamar and Fahrenfeld, N L
Journal: The Science of the total environment (2020): 138865
The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils.
Authors: Prajsnar, Tomasz K and Serba, Justyna J and Dekker, Bernice M and Gibson, Josie F and Masud, Samrah and Fleming, Angeleen and Johnston, Simon A and Renshaw, Stephen A and Meijer, Annemarie H
Journal: Autophagy (2020): 1-15
A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining.
Authors: Antonov, Stanislav A and Novosadova, Ekaterina V and Kobylansky, Andrey G and Tarantul, Vyacheslav Z and Grivennikov, Igor A
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2019): 771-782
说明书
ReadiUse™Tyramide（TSA）/ Styramide（PSA）优化的反应缓冲液.pdf
