标签归档：载体

pCMV-Tag 2A载体说明书

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型号 载体名称 出品公司 载体用途 价格
VPS0039 pCMV-Tag 2A Stratagene 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：CMV
Cloning Method：Restriction Enzyme/MCS
Constitutive or Inducible System：Constitutive

载体抗性: 

卡那霉素（Kanamycin）

载体描述: 

Epitope tags provide a method to localize gene products in living cells, identify associated proteins, track the movement of tagged proteins within a cell, and characterize new genes without creating protein-specific antibodies. The pCMV-Tag1 vector contains both the synthetic FLAG epitopell ll and the c-myc epitope from the human c-myc gene. The pCMV-Tag1 vector allows production of fusion proteins in a variety of conformations. The pCMV-Tag2-5 vectors allow either c-myc or FLAG epitope tagging at either the C- or N-terminus. Each pCMV-Tag2-5 vector is supplied with all three reading frames for easy cloning and expression. The small size of the FLAG （eight amino acids long） and c-myc epitope tags （ten amino acids long） decreases the possibility of interference with the tagged protein. The FLAG and c-myc epitopes are recognized by the anti-c-myc and anti-FLAG antibodies, which can be used to characterize the target protein. The pCMV-Tag vectors allow high-level expression of tagged proteins in mammalian cells. Expression is driven by the human cytomegalovirus （CMV） immediate early promoter. The CMV promoter provides constitutive expression of cloned genes in a wide variety of cell lines. The translational start sequence used in the pCMV-Tag1, pCMV-Tag2 and pCMV-Tag3 vectors is a 10-base Kozak consensus sequence of CGG（A/G）CCATGG. pCMV-Tag4 and pCMV-Tag5 vectors do not contain a translation start sequence. Stable clone selection is made possible with G418 by the presence of the neomycin-resistance gene. The pCMV-Tag vectors are only 4.3 kb, allowing easy cloning and vector manipulations. This small size is due to the single antibiotic selection marker used by both prokaryotic and mammalian cells. The neomycin-resistance gene provides stable selection in mammalian cells with G418 driven by the SV40 promoter and kanamycin selection in E. coli cells driven by the bla （ß-lactamase） promoter.

 

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pEF1/myc-His C载体说明书

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型号 载体名称 出品公司 载体用途 价格
VPI0028 pEF1/myc-His C Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 

pEF1/myc-His B载体说明书

发表于由

型号 载体名称 出品公司 载体用途 价格
VPI0027 pEF1/myc-His B Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 

pEF1/myc-His A载体说明书

发表于由

型号 载体名称 出品公司 载体用途 价格
VPI0026 pEF1/myc-His A Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 

pTracer CMV2载体说明书

发表于由

 

pTracer CMV2

型号 载体名称 出品公司 载体用途
VPI0016 pTracer CMV2 Invitrogen 哺乳动物表达载体
产品参数: 

Mammalian Selection：Zeocin
Marker：GFP

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

这是一个双表达框载体，CMV启动子后有MCS供插入外源基因，hEF1α启动子调控Cycle 3 GFP-Zeocin™ fusion的表达，可以做到既有荧光蛋白来做转染指示，且荧光基因表达和外源基因表达又不会互相干扰，而且Zeocin也可以用来做稳定性细胞筛选。另外，由于EM-7启动子的存在，Cycle 3 GFP-Zeocin™ fusion在E.coli中也可以表达，可以用来做原核荧光定位。

Three pTracer™ mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin™ . Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin™ fusion.

All three pTracer™ vectors have the following features:

  • Cycle 3 GFP-Zeocin™ fusion for selection in mammalian cell lines
  • BGH polyA signal and transcription termination sequences to enhance mRNA stability

The pTracer™-SV40 vector offers:

  • SV40 promoter for high-level constitutive expression of your gene of interest
  • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigen

The pTracer™-CMV2 vector offers:

  • CMV promoter for high-level constitutive expression of your gene of interest
  • EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
  • Ampicillin resistance gene for selection in E. coli

The pTracer™-EF vectors offer:

  • EF-1α promoter for high-level constitutive expression of your gene of interest
  • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
  • C-terminal V5 epitope tag for simple detection of recombinant fusion proteins with an Anti-V5 Antibody
  • C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His（C-term） Antibody
  • Multiple cloning site supplied in three reading frames to simplify cloning in frame with the C-terminal tag
质粒图谱: 

pBudCE4.1载体说明书

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pBudCE4.1

型号 载体名称 出品公司 载体用途
VPI0014 pBudCE4.1 Invitrogen 哺乳动物表达载体
产品参数: 

Mammalian Selection：Zeocin
Sequencing Primer：CMV-f、hEF1α-f
Tag：6X His，myc

载体抗性: 
博莱霉素（Zeocin）
载体描述: 

质粒特点：双表达框，两个强启动子（CMV启动子和hEF1α启动子）各负责一个，不会互相干扰，从而可以实现同时表达两个基因，且两者表达不互相影响的目的。如果在hEF1α启动子后面插入EGFP或者其它荧光基因，CMV启动子后插入外源基因，则可以做到既有荧光蛋白来做转染指示，且荧光基因表达和外源基因表达又不会互相干扰。Yrbio已经构建了pBudCE4.1-EGFP、pBudCE4.1-RFP、pBudCE4.1-YFP等质粒，客户可直接购买。

质粒图谱: 

pVAX1载体说明书

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pVAX1

型号 载体名称 出品公司 载体用途
VPI0038 pVAX1 Invitrogen 哺乳动物表达载体
产品参数: 

Mammalian Selection：-
Sequencing Primer：T7 Fwd
Sequencing Primer Sequence：5’d[TAATACGACTCACTATAGGG]3′

载体抗性: 
卡那霉素（Kanamycin）
载体描述: 

pVAX1是在载体pcDNA3.1的基础上改建而成的一种新型真核表达载体，大小为3.0 kb。该质粒用卡那霉素抗性筛选基因替代pcDNA3.1中的氨卞霉素抗性筛选基因，zui大程度地减少抗性筛选基因和人类基因组发生重组的可能性。pVAX1含有多个克隆酶切位点允许同时克隆多个大片段的目的基因，强启动子pCMV和BGH poly A信号可以在哺乳动物细胞中高水平表达重组蛋白。pVAX1又是一种非融合载体，要求插入序列中时必须包含一段KozaK的翻译起始序列和一个起始密码子（ATG）以引导正确的翻译，典型的KozaK共有序列为“ANNATGG”，其-3位的A和+4位的G对正确插入甚为关键，在插入序列中还需包括一个终止密码子以正确终止基因表达。

pVAX1是由美国食品和药品管理委员会（FDA）推荐的*可以应用于人体实验的载体质粒。迄今为止，关于pVAX1的研究已涉及动物和临床试验。

质粒图谱: 

pcDNA3.1（hyg）+载体说明书

发表于由

 

pcDNA3.1（hyg）+

型号 载体名称 出品公司 载体用途
VPI0008 pcDNA3.1（hyg）+ Invitrogen 哺乳动物表达载体

 

产品参数: 

Promotor: CMV
5′ Sequencing 1 Primer: T7 Fwd
5′ Sequencing 1 Primer Sequence: 5’d[TAATACGACTCACTATAGGG]3′
Selectable Marker: Hygromycin

载体描述: 

Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.

说明书: 
 pcDNA3.1（hyg）+ 载体序列
质粒图谱: 

pCo hygro载体说明书

发表于由

 

pCo hygro

型号 载体名称 出品公司 载体用途
VGI0071 pCo hygro Invitrogen 果蝇细胞表达系统

Comments for pCoHygro

4526 nucleotides

copia promoter: bases 500-746

Hygromycin resistance gene: bases 781-1830

SV40 early polyadenylation sequence: bases 2146-2280

pUC origin: bases 2648-3321 （complementary strand）

Ampicillin （bla） resistance gene: bases 3466-4326 （complementary strand）

bla promoter: bases 4327-4425 （complementary strand）

质粒图谱: 

pDisplay载体说明书

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pDisplay

型号 载体名称 出品公司 载体用途
VBI0471 pDisplay Invitrogen 哺乳动物表面展示系统
产品参数: 

Mammalian Selection：Neomycin，G418
Host：mammalian
N/C Term：C terminus
MCS：SfiI-BglII-XmaI-SmaI-SacII-PstI-SalI-AccI

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pDisplay™ is a mammalian expression vector designed to target recombinant proteins to the surface of mammalian cells. Displayed proteins can be analyzed for their ability to interact with known or putative ligands. Proteins of interest are targeted and anchored to the cell surface by cloning the gene of interest in frame with the vectors unique N-terminal secretion signal and the C-terminal transmembrane anchoring domain of plaet-derived growth factor receptor （PDGFR）. In contrast to phage display vectors which operate exclusively in prokaryotic cells, the pDisplay™ Vector offers the advantage of having the displayed protein of interest processed in mammalian cells. Therefore, recombinant proteins of eukaryotic origin that are expressed from pDisplay™ more closely resemble their native form. This may favor more accurate ligand binding interactions. Inaddition to the N-terminal cell surface targeting signal and C-terminal transmembrane anchoring domain, the pDisplay™ Vector contains the following key features:

  1. T7 promoter/priming site for in vitro transcription of sense RNA and for sequencing of inserts
  2. Neomycin resistance marker for stable selection in mammalian cells using Geneticin®
  3. SV40 origin for replication and simple vector rescue in cell lines expressing the large T antigen （e.g., COS-1 and COS-7）
  4. Ampicillin resistance gene for selection in E. coli
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EBY100载体说明书

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EBY100

型号 载体名称 出品公司 载体用途
VNI0473 EBY100 Invitrogen 配套酿酒酵母

Contents 20 µg of pYD1, lyophilized in TE, pH 8 

EBY100 glycerol stock, 0.5 ml 

2 µg pYD1 Forward sequencing primer, lyophilized 

2 µg pYD1 Reverse sequencing primer, lyophilized 

Shipping/Storage The pYD1 Vector Kit is shipped on dry ice. Upon receipt: 

• Store the glycerol stock at -80°C 

• Store the lyophilized plasmid and primers at -20°C 

 

DB3.1载体说明书

发表于由

 

DB3.1

型号 载体名称 出品公司 载体用途
VDI0537 DB3.1 Invitrogen 克隆菌株

 

Description:

LIBRARY EFFICIENCY DB3.1 Competent Cells have been prepared by a

patented modification of the procedure of Hanahan （1）. These cells contain

the gyrA462 allele which renders the strain resistant to the toxic effects of the

ccdB gene （2）. LIBRARY EFFICIENCY DB3.1 Competent Cells can be used for

propagating plasmids containing the ccdB gene such as GATEWAY System

vectors or for constructing new plasmids containing the ccdB gene.

Pichiapink（strain 1-wildtype）载体说明书

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Pichiapink（strain 1-wildtype）

型号 载体名称 出品公司 载体用途
VBI0309 Pichiapink（strain 1-wildtype） Invitrogen 配套毕赤酵母

The PichiaPink™ Secretion Signal Kit, included in the PichiaPink™ Secretion 

Optimization Kit, is also available separay from Invitrogen （Cat. no. A11155）. 

The PichiaPink™ Secretion Signal Kit consists of eight secretion signal sequences 

supplied as phosphorylated duplexes in 40 pmol lyophilized aliquots. The 

secretion signals and their sources are listed below. Upon receipt, store the 

secretion signal duplexes at –20°C.

SMD1163载体说明书

发表于由

 

SMD1163

型号 载体名称 出品公司 载体用途
VBI0307 SMD1163 Invitrogen 配套毕赤酵母

Introduction 

Pichia pastoris is a highly successful system for production of a wide variety of 

recombinant proteins. Several factors have contributed to its rapid acceptance, the most 

important of which include: （1） a promoter derived from the alcohol oxidase I （AOX1） gene of P. 

pastoris that is uniquely suited for the controlled expression of foreign genes; （2） the similarity 

of techniques needed for the molecular genetic manipulation of P. pastoris to those of 

Saccharomyces cerevisiae; （3） the strong preference of P. pastoris for respiratory growth, a key 

physiological trait that greatly facilitates its culturing at high-cell densities relative to 

fermentative yeasts; and （4） the decision in 1993 by Phillips Petroleum Company in Bartlesville, 

Okla. （and continued by Research Corporation Technologies [RCT]）, to release the P. pastoris

expression system to academic research laboratories, the consequence of which has been an 

explosion in the knowledge base on the system as described in numerous recent publications.