-
- 产品货号:
- 4485064
-
- 中文名称:
- MLG TAQMAN STEC O26 & O111 BEADS
-
- 品牌:
- OXOID
-
货号
产品规格
售价
备注
-
4485064-96tests
96tests
¥13234.30
食品安全小型设备&试剂盒
产品描述
货号
产品规格
售价
备注
4485064-96tests
96tests
¥13234.30
食品安全小型设备&试剂盒
产品描述
货号
产品规格
售价
备注
4485065-96tests
96tests
¥13234.30
食品安全小型设备&试剂盒
产品描述
货号
产品规格
售价
备注
4485066-96tests
96tests
¥13234.30
食品安全小型设备&试剂盒
产品描述
上海金畔生物科技有限公司提供2×Taqman PCR MasterMix,索莱宝,SR2110-200T,可以访问官网了解更多产品信息。
2×Taqman PCR MasterMix,索莱宝,SR2110-200T
· 别名 : 实时荧光定量试剂盒 DNA荧光定量试剂盒
· 英文名称 : 2×Taqman PCR MasterMix
· 储存条件 : -20℃
· 单位 : 盒
用于分子生物学科研实验研究。
浓度 | |
规格 | 200T |
保存 | -20℃ |
上海金畔生物科技有限公司提供2×Taqman PCR MasterMix,索莱宝,SR2110-50T,可以访问官网了解更多产品信息。
2×Taqman PCR MasterMix,索莱宝,SR2110-50T
· 别名 : 实时荧光定量试剂盒 DNA荧光定量试剂盒
· 英文名称 : 2×Taqman PCR MasterMix
· 储存条件 : -20℃
· 单位 : 盒
用于分子生物学科研实验研究。
浓度 | |
规格 | 50T |
保存 | -20℃ |
上海金畔生物科技有限公司提供TaqMan One Step RT-qPCR Kit,索莱宝,T2210-200T,可以访问官网了解更多产品信息。
TaqMan One Step RT-qPCR Kit,索莱宝,T2210-200T
· 别名 : Taqman一步法荧光定量PCR试剂盒 一步法实时荧光定量试剂盒
· 英文名称 : TaqMan One Step RT-qPCR Kit
· 储存条件 : -20℃
· 单位 : 支
本产品是采用探针法(TaqMan,Molecular Beacon等)进行一步法Real-Time RTqPCR的专用试剂盒。使用本产品进行RealTime RT-qPCR反应时,逆转录和定量PCR在同一反应体系中进行,反应过程中无需添加试剂,无需打开管盖,避免了污染的同时提高了实验效率。本产品的检测灵敏度高,荧光信号强,信噪比高,非常适合于RNA病毒等微量RNA的检测。其所包含的特殊缓冲系统能使逆转录酶与DNA聚合酶同时发挥最大功效,提高反应效率。使用本产品可以得到更宽广的线性范围,对目的基因定量更准确,重复性好,可信度高。
浓度 | |
规格 | 200T |
保存 | -20℃ |
上海金畔生物科技有限公司提供TaqMan One Step RT-qPCR Kit,索莱宝,T2210-50T,可以访问官网了解更多产品信息。
TaqMan One Step RT-qPCR Kit,索莱宝,T2210-50T
· 别名 : Taqman一步法荧光定量PCR试剂盒 一步法实时荧光定量试剂盒
· 英文名称 : TaqMan One Step RT-qPCR Kit
· 储存条件 : -20℃
· 单位 : 支
本产品是采用探针法(TaqMan,Molecular Beacon等)进行一步法Real-Time RTqPCR的专用试剂盒。使用本产品进行RealTime RT-qPCR反应时,逆转录和定量PCR在同一反应体系中进行,反应过程中无需添加试剂,无需打开管盖,避免了污染的同时提高了实验效率。本产品的检测灵敏度高,荧光信号强,信噪比高,非常适合于RNA病毒等微量RNA的检测。其所包含的特殊缓冲系统能使逆转录酶与DNA聚合酶同时发挥最大功效,提高反应效率。使用本产品可以得到更宽广的线性范围,对目的基因定量更准确,重复性好,可信度高。
浓度 | |
规格 | 50T |
保存 | -20℃ |
上海金畔生物科技有限公司提供SGExcel GoldStar TaqMan Master 预混液(含 ROX),生工,B532953-0001 1ml,可以访问官网了解更多产品信息。
SGExcel GoldStar TaqMan Master 预混液(含 ROX),生工,B532953-0001 1ml
英文名 | SGExcel GoldStar TaqMan Mixture (with ROX) | ||
相关类别 | 荧光定量PCR(Real-time PCR) | 储存 | 冷冻(-20℃) 避光 |
|
SGExcel GoldStar TaqMan Mixture(With ROX)是专用于探针法(TaqMan, Molecular Beacon等)实时荧光定量PCR的预混体系,浓度为2×,包含GoldStar Taq DNA Polymerase、PCR Buffer、dNTPs、Mg2+和ROX,操作简单方便。主要用于基因组DNA靶序列和RNA反转录后cDNA靶序列检测,如基因表达分析,拷贝数分析,SNP基因型分析等,适用于不同类型探针法荧光定量。
基因表达分析、拷贝数分析、SNP分析
2×SGExcel GoldStar TaqMan Mixture(With ROX)
浓度 | |
规格 | 1ml |
保存 | 冷冻(-20℃) 避光 |
上海金畔生物科技有限公司提供SGExcel GoldStar TaqMan Master 预混液(含 ROX),生工,B532953-0005 5ml,可以访问官网了解更多产品信息。
SGExcel GoldStar TaqMan Master 预混液(含 ROX),生工,B532953-0005 5ml
英文名 | SGExcel GoldStar TaqMan Mixture (with ROX) | ||
相关类别 | 荧光定量PCR(Real-time PCR) | 储存 | 冷冻(-20℃) 避光 |
|
SGExcel GoldStar TaqMan Mixture(With ROX)是专用于探针法(TaqMan, Molecular Beacon等)实时荧光定量PCR的预混体系,浓度为2×,包含GoldStar Taq DNA Polymerase、PCR Buffer、dNTPs、Mg2+和ROX,操作简单方便。主要用于基因组DNA靶序列和RNA反转录后cDNA靶序列检测,如基因表达分析,拷贝数分析,SNP基因型分析等,适用于不同类型探针法荧光定量。
基因表达分析、拷贝数分析、SNP分析
2×SGExcel GoldStar TaqMan Mixture(With ROX)
浓度 | |
规格 | 5ml |
保存 | 冷冻(-20℃) 避光 |
上海金畔生物科技有限公司提供SGExcel GoldStar TaqMan Master 预混液,生工,B532932-0001 1ml,可以访问官网了解更多产品信息。
SGExcel GoldStar TaqMan Master 预混液,生工,B532932-0001 1ml
英文名 | SGExcel GoldStar TaqMan Mixture | ||
相关类别 | 荧光定量PCR(Real-time PCR) | 储存 | 冷冻(-20℃) 避光 |
|
SGExcel GoldStar TaqMan Mixture是专用于探针法(TaqMan, Molecular Beacon等)实时荧光定量PCR的预混体系,浓度为2×,包含GoldStar Taq DNA Polymerase、PCR Buffer、dNTPs和Mg2+ ,操作简单方便。主要用于基因组DNA靶序列和RNA反转录后cDNA靶序列检测,如基因表达分析,拷贝数分析,SNP基因型分析等,适用于不同类型探针法荧光定量。
基因表达分析、拷贝数分析、SNP分析
2×SGExcel GoldStar TaqMan Mixture
浓度 | |
规格 | 1ml |
保存 | 冷冻(-20℃) 避光 |
上海金畔生物科技有限公司提供SGExcel GoldStar TaqMan Master 预混液,生工,B532932-0005 5ml,可以访问官网了解更多产品信息。
SGExcel GoldStar TaqMan Master 预混液,生工,B532932-0005 5ml
英文名 | SGExcel GoldStar TaqMan Mixture | ||
相关类别 | 荧光定量PCR(Real-time PCR) | 储存 | 冷冻(-20℃) 避光 |
|
SGExcel GoldStar TaqMan Mixture是专用于探针法(TaqMan, Molecular Beacon等)实时荧光定量PCR的预混体系,浓度为2×,包含GoldStar Taq DNA Polymerase、PCR Buffer、dNTPs和Mg2+ ,操作简单方便。主要用于基因组DNA靶序列和RNA反转录后cDNA靶序列检测,如基因表达分析,拷贝数分析,SNP基因型分析等,适用于不同类型探针法荧光定量。
基因表达分析、拷贝数分析、SNP分析
2×SGExcel GoldStar TaqMan Mixture
浓度 | |
规格 | 5ml |
保存 | 冷冻(-20℃) 避光 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 17283 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 5 mL | 价格 | 3612 | |
Ex (nm) | Em (nm) | |||
分子量 | 溶剂 | Water | ||
产品详细介绍 |
简要概述
产品基本信息
货号:17283
产品名称:TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*
规格:5ml
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
溶剂:水
产品介绍
用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液,针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分,包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP,但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型(如基因组、质粒、病毒和 cDNA 模板)的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*。
适用仪器
qPCR | |
仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
成分 | 体积 (25 µL/reaction) | 体积 (50 µL/reaction) | 最终浓度 |
TAQuest qPCR Master Mix 用于TaqMan探针*无ROX* | 12.5 µL | 25 µL | 1X |
上游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
下游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
DNA模板 | 1-5 µL | 1-5 µL | 优化的浓度 |
无核酸酶水 | 25 µL | 50 µL |
表 2. 热循环参数
范围 | 聚合酶激活 | PCR (30-40个循环) | ||
Hold | 变性 | 退火 | 延伸 | |
温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples.
Authors: Pereira-Gómez, Marianoel and Fajardo, Álvaro and Echeverría, Natalia and López-Tort, Fernando and Perbolianachis, Paula and Costábile, Alicia and Aldunate, Fabián and Moreno, Pilar and Moratorio, Gonzalo
Journal: Journal of virological methods (2021): 114035
Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR.
Authors: Dorlass, Erick Gustavo and Monteiro, Cairo Oliveira and Viana, Amanda Oliveira and Soares, Camila Pereira and Machado, Rafael Rahal Guaragna and Thomazelli, Luciano Matsumiya and Araujo, Danielle Bastos and Leal, Fabyano Bruno and Candido, Erika Donizette and Telezynski, Bruna Larotonda and Valério, Camila Araujo and Chalup, Vanessa Nascimento and Mello, Ralyria and Almeida, Flavia Jaqueline and Aguiar, Andressa Simões and Barrientos, Anna Carlotta Mott and Sucupira, Carolina and De Paulis, Milena and Sáfadi, Marco Aurélio Palazzi and Silva, Daniella Gregorio Bonfim Prado and Sodré, Janaina Joice Martins and Soledade, Mariana Pereira and Matos, Samantha Faria and Ferreira, Sabrina Rodrigues and Pinez, Célia Miranda Nunez and Buonafine, Carolina Palamin and Pieroni, Leticia Nery Ferreira and Malta, Fernanda Mello and Santana, Rubia Anita Ferraz and Souza, Eloisa Corrêa and Fock, Ricardo Ambrosio and Pinho, João Renato Rebelo and Ferreira, Luís Carlos Souza and Botosso, Viviane Fongaro and Durigon, Edison Luiz and Oliveira, Danielle Bruna Leal
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2020): 1117-1123
Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB.
Authors: Cruz-Flores, Roberto and Mai, Hung Nam and Dhar, Arun K
Journal: Molecular and cellular probes (2019): 20-28
Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.
Authors: Peng, Xiujuan and Nguyen, Alex and Ghosh, Debadyuti
Journal: Journal of virological methods (2018): 100-107
sjTREC quantification using SYBR quantitative PCR for age estimation of bloodstains in a Japanese population.
Authors: Yamanoi, Eisuke and Uchiyama, Saori and Sakurada, Makoto and Ueno, Yasuhiro
Journal: Legal medicine (Tokyo, Japan) (2018): 71-74
Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus.
Authors: Zhou, Xinrong and Zhang, Tiansheng and Song, Deping and Huang, Tao and Peng, Qi and Chen, Yanjun and Li, Anqi and Zhang, Fanfan and Wu, Qiong and Ye, Yu and Tang, Yuxin
Journal: Molecular and cellular probes (2017): 36-41
Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic.
Authors: Gomes, Ciro Martins and Cesetti, Mariana Vicente and de Paula, Natália Aparecida and Vernal, Sebastián and Gupta, Gaurav and Sampaio, Raimunda Nonata Ribeiro and Roselino, Ana Maria
Journal: Journal of clinical microbiology (2017): 526-534
Qualitative Sybr Green real-time detection of single nucleotide polymorphisms responsible for target-site resistance in insect pests: the example of Myzus persicae and Musca domestica.
Authors: Puggioni, V and Chiesa, O and Panini, M and Mazzoni, E
Journal: Bulletin of entomological research (2017): 96-105
Sybr Green- and TaqMan-Based Quantitative PCR Approaches Allow Assessment of the Abundance and Relative Distribution of Frankia Clusters in Soils.
Authors: Ben Tekaya, Seifeddine and Ganesan, Abirama Sundari and Guerra, Trina and Dawson, Jeffrey O and Forstner, Michael R J and Hahn, Dittmar
Journal: Applied and environmental microbiology (2017)
An Evaluation of Quantitative PCR Assays (TaqMan® and SYBR Green) for the Detection of Babesia bigemina and Babesia bovis, and a Novel Fluorescent-ITS1-PCR Capillary Electrophoresis Method for Genotyping B. bovis Isolates.
Authors: Zhang, Bing and Sambono, Jacqueline L and Morgan, Jess A T and Venus, Bronwyn and Rolls, Peter and Lew-Tabor, Ala E
Journal: Veterinary sciences (2016)
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 17284 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 1 mL | 价格 | 1164 | |
Ex (nm) | Em (nm) | |||
分子量 | 溶剂 | Water | ||
产品详细介绍 |
简要概述
产品基本信息
货号:17284
产品名称:TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*
规格:1ml
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
溶剂:水
产品介绍
用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液,针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分,包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP,但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型(如基因组、质粒、病毒和 cDNA 模板)的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*。
适用仪器
qPCR | |
仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
成分 | 体积 (25 µL/reaction) | 体积 (50 µL/reaction) | 最终浓度 |
TAQuest qPCR Master Mix 用于TaqMan探针*低ROX* | 12.5 µL | 25 µL | 1X |
上游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
下游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
DNA模板 | 1-5 µL | 1-5 µL | 优化的浓度 |
无核酸酶水 | 25 µL | 50 µL |
表 2. 热循环参数
范围 | 聚合酶激活 | PCR (30-40个循环) | ||
Hold | 变性 | 退火 | 延伸 | |
温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813
Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5
Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9
[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6
[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61
[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 17286 | 存储条件 | 在零下15度以下保存, 避免光照 |
规格 | 1 mL | 价格 | 1164 |
Ex (nm) | Em (nm) | ||
分子量 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:17286
产品名称:TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*
规格:1ml
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
溶剂:水
产品介绍
用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液,针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分,包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP,但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型(如基因组、质粒、病毒和 cDNA 模板)的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。
适用仪器
qPCR | |
仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
成分 | 体积 (25 µL/reaction) | 体积 (50 µL/reaction) | 最终浓度 |
TAQuest qPCR Master Mix 用于TaqMan探针*高ROX* | 12.5 µL | 25 µL | 1X |
上游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
下游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
DNA模板 | 1-5 µL | 1-5 µL | 优化的浓度 |
无核酸酶水 | 25 µL | 50 µL |
表 2. 热循环参数
范围 | 聚合酶激活 | PCR (30-40个循环) | ||
Hold | 变性 | 退火 | 延伸 | |
温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813
Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5
Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9
[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6
[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61
[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 17282 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 1 mL | 价格 | 1164 | |
Ex (nm) | Em (nm) | |||
分子量 | 溶剂 | Water | ||
产品详细介绍 |
简要概述
产品基本信息
货号:17282
产品名称:TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*
规格:1ml
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
溶剂:水
产品介绍
用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液,针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分,包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP,但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型(如基因组、质粒、病毒和 cDNA 模板)的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*。
适用仪器
qPCR | |
仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
成分 | 体积 (25 µL/reaction) | 体积 (50 µL/reaction) | 最终浓度 |
TAQuest qPCR Master Mix 用于TaqMan探针*无ROX* | 12.5 µL | 25 µL | 1X |
上游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
下游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
DNA模板 | 1-5 µL | 1-5 µL | 优化的浓度 |
无核酸酶水 | 25 µL | 50 µL |
表 2. 热循环参数
范围 | 聚合酶激活 | PCR (30-40个循环) | ||
Hold | 变性 | 退火 | 延伸 | |
温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples.
Authors: Pereira-Gómez, Marianoel and Fajardo, Álvaro and Echeverría, Natalia and López-Tort, Fernando and Perbolianachis, Paula and Costábile, Alicia and Aldunate, Fabián and Moreno, Pilar and Moratorio, Gonzalo
Journal: Journal of virological methods (2021): 114035
Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR.
Authors: Dorlass, Erick Gustavo and Monteiro, Cairo Oliveira and Viana, Amanda Oliveira and Soares, Camila Pereira and Machado, Rafael Rahal Guaragna and Thomazelli, Luciano Matsumiya and Araujo, Danielle Bastos and Leal, Fabyano Bruno and Candido, Erika Donizette and Telezynski, Bruna Larotonda and Valério, Camila Araujo and Chalup, Vanessa Nascimento and Mello, Ralyria and Almeida, Flavia Jaqueline and Aguiar, Andressa Simões and Barrientos, Anna Carlotta Mott and Sucupira, Carolina and De Paulis, Milena and Sáfadi, Marco Aurélio Palazzi and Silva, Daniella Gregorio Bonfim Prado and Sodré, Janaina Joice Martins and Soledade, Mariana Pereira and Matos, Samantha Faria and Ferreira, Sabrina Rodrigues and Pinez, Célia Miranda Nunez and Buonafine, Carolina Palamin and Pieroni, Leticia Nery Ferreira and Malta, Fernanda Mello and Santana, Rubia Anita Ferraz and Souza, Eloisa Corrêa and Fock, Ricardo Ambrosio and Pinho, João Renato Rebelo and Ferreira, Luís Carlos Souza and Botosso, Viviane Fongaro and Durigon, Edison Luiz and Oliveira, Danielle Bruna Leal
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2020): 1117-1123
Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB.
Authors: Cruz-Flores, Roberto and Mai, Hung Nam and Dhar, Arun K
Journal: Molecular and cellular probes (2019): 20-28
Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.
Authors: Peng, Xiujuan and Nguyen, Alex and Ghosh, Debadyuti
Journal: Journal of virological methods (2018): 100-107
sjTREC quantification using SYBR quantitative PCR for age estimation of bloodstains in a Japanese population.
Authors: Yamanoi, Eisuke and Uchiyama, Saori and Sakurada, Makoto and Ueno, Yasuhiro
Journal: Legal medicine (Tokyo, Japan) (2018): 71-74
Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus.
Authors: Zhou, Xinrong and Zhang, Tiansheng and Song, Deping and Huang, Tao and Peng, Qi and Chen, Yanjun and Li, Anqi and Zhang, Fanfan and Wu, Qiong and Ye, Yu and Tang, Yuxin
Journal: Molecular and cellular probes (2017): 36-41
Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic.
Authors: Gomes, Ciro Martins and Cesetti, Mariana Vicente and de Paula, Natália Aparecida and Vernal, Sebastián and Gupta, Gaurav and Sampaio, Raimunda Nonata Ribeiro and Roselino, Ana Maria
Journal: Journal of clinical microbiology (2017): 526-534
Qualitative Sybr Green real-time detection of single nucleotide polymorphisms responsible for target-site resistance in insect pests: the example of Myzus persicae and Musca domestica.
Authors: Puggioni, V and Chiesa, O and Panini, M and Mazzoni, E
Journal: Bulletin of entomological research (2017): 96-105
Sybr Green- and TaqMan-Based Quantitative PCR Approaches Allow Assessment of the Abundance and Relative Distribution of Frankia Clusters in Soils.
Authors: Ben Tekaya, Seifeddine and Ganesan, Abirama Sundari and Guerra, Trina and Dawson, Jeffrey O and Forstner, Michael R J and Hahn, Dittmar
Journal: Applied and environmental microbiology (2017)
An Evaluation of Quantitative PCR Assays (TaqMan® and SYBR Green) for the Detection of Babesia bigemina and Babesia bovis, and a Novel Fluorescent-ITS1-PCR Capillary Electrophoresis Method for Genotyping B. bovis Isolates.
Authors: Zhang, Bing and Sambono, Jacqueline L and Morgan, Jess A T and Venus, Bronwyn and Rolls, Peter and Lew-Tabor, Ala E
Journal: Veterinary sciences (2016)
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 17285 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 5 mL | 价格 | 3612 | |
Ex (nm) | Em (nm) | |||
分子量 | 溶剂 | Water | ||
产品详细介绍 |
简要概述
产品基本信息
货号:17285
产品名称:TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*
规格:5ml
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
溶剂:水
产品介绍
用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液,针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分,包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP,但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型(如基因组、质粒、病毒和 cDNA 模板)的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*。
适用仪器
qPCR | |
仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
成分 | 体积 (25 µL/reaction) | 体积 (50 µL/reaction) | 最终浓度 |
TAQuest qPCR Master Mix 用于TaqMan探针*低ROX* | 12.5 µL | 25 µL | 1X |
上游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
下游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
DNA模板 | 1-5 µL | 1-5 µL | 优化的浓度 |
无核酸酶水 | 25 µL | 50 µL |
表 2. 热循环参数
范围 | 聚合酶激活 | PCR (30-40个循环) | ||
Hold | 变性 | 退火 | 延伸 | |
温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813
Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5
Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9
[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6
[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61
[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 17287 | 存储条件 | 在零下15度以下保存, 避免光照 |
规格 | 5 mL | 价格 | 3612 |
Ex (nm) | Em (nm) | ||
分子量 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:17287
产品名称:TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*
规格:5ml
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
溶剂:水
产品介绍
用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液,针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分,包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP,但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型(如基因组、质粒、病毒和 cDNA 模板)的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。
适用仪器
qPCR | |
仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
成分 | 体积 (25 µL/reaction) | 体积 (50 µL/reaction) | 最终浓度 |
TAQuest qPCR Master Mix 用于TaqMan探针*高ROX* | 12.5 µL | 25 µL | 1X |
上游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
下游引物,10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
DNA模板 | 1-5 µL | 1-5 µL | 优化的浓度 |
无核酸酶水 | 25 µL | 50 µL |
表 2. 热循环参数
范围 | 聚合酶激活 | PCR (30-40个循环) | ||
Hold | 变性 | 退火 | 延伸 | |
温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813
Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
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Journal: BioTechniques (2014): 151-5
Development of a novel internal positive control for Taqman based assays.
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[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
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[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61
[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
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