5′ sequencing primer: T7

Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.

Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus （VSV-G） under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus（MMLV）-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipidcomponents of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein. 
The vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria.