nkmaxbio AAD0614说明书

nkmaxbio AAD0614说明书

nkmaxbio AAD0614说明书

Human Adiponectin/Acrp30 antibody

人脂联素/Acrp30抗体

  • Cat No.

  • AAD0614

产品类型

单克隆抗体

克隆编号5H7

UniProt编号:Q15848

NCBI登录号NP_004788

备选名称ADIPOQ、ACDC、ACRP30、APM1、GBP28、脂联素球状结构域、小鼠、脂肪细胞、C1q和含胶原蛋白结构域的蛋白质、30kDa脂肪细胞补体相关蛋白质、脂肪细胞补补体相关30kDa蛋白、ACRP40、脂肪丰富、脂联蛋白、30kda脂肪细胞补体相关蛋白质,ACRP 30、脂肪细胞C1q和含有胶原蛋白结构的蛋白质,脂肪细胞补体相关30kDa蛋白,脂肪细胞补补体相关蛋白30kDa,脂肪丰富的基因转录物1,APM1,GBP28,明胶结合蛋白

与人类反应

浓度1mg/ml(通过BCA测定)

配方含0.02%叠氮化纳、10%甘油的磷酸盐缓冲盐水(pH 7.4)液体

免疫原从大肠杆菌中纯化的重组人脂联素(15-244aa)

同种型IgG1κ

蛋白G亲和层析纯化

应用ELISA、WB、ICC/IF

用途通过ELISA、Western blot和ICC/IF分析测试抗体,以确保特异性和反应性。然而,由于应用不同,每次调查都应该用试剂滴定,以获得最佳结果。

存储可在+2C至+8C温度下存储1周。对于长期储存,等分并储存在-20℃至-80℃。避免重复冷冻和解冻循环。

    Immunflorescense (ICC/IF)

    ICC/IF analysis of Adiponectin in Hep3B cells. The cell was stained with AAD0614 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

    Western blot analysis (WB)

    The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
    Lane 1. : 293T cell lysate
    Lane 2. : Adiponectin collagen domain(15-107aa) transfected 293T cell lysate
    Lane 3. : Adiponectin Full domain(15-244aa) transfected 293T cell lysate