Anti-CCSP-Rabbit (WRAB-3950)使用说明

Anti-CCSP-Rabbit (WRAB-3950)使用说明

Anti-CCSP-Rabbit (WRAB-3950)使用说明

产品信息

   

Seven Hills Bioreagents

   

WRAB-3950

   

100 µL

宿    

反应种属

人、鼠

克隆类型

多克隆

0.01% sodium azide

   

重组鼠CCSP融合6*his蛋白

保存条件

储存在-20,避免反复冻融

蛋白背景

Clara细胞分泌蛋白(CCSP)是Clara细胞最主要的分泌物,由二条相同肽链反向排列连接而成,CCSP作为肺上皮特异性蛋白已成为肺上皮屏障损伤的外周标志。CCSP具有抗炎、抗氧化、免疫调节、肿瘤抑制等多种生物活性。

 

产品使用

实验应用

建议稀释倍数

Western Blot

1:10000

Immunohistochemistry

1:20000

说明:最佳稀释倍数与浓度应由研究人员确认

应用实例

 

图片用抗CCSP兔血清(1:20000稀释液)染色的成年小鼠肺组织。CCSP通过DAB(黑色)进行可视化,并用Nuclear Fast Red(粉红色)进行复染。放大倍数20

参考文献

1. Harrod, KS, Mounday, AD, Stripp, BR, Whitsett, JA. 'Clara cell secretory protein decreases lung inflammation after acute virus infection.' Am J Physiol. 1998 Nov;275(5 Pt 1):L924-30.

2. Stripp BR, Lund J, Mango GW, Doyen KC, Johnston C, Hultenby K, Nord M, Whitsett JA. 'Clara cell secretory protein: a determinant of PCB bioaccumulation in mammals.' Am J Physiol. 1996 Oct;271(4 Pt 1):L656-64.

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