progen 61055现货

progen 61055现货

progen 61055现货

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

抗AAV2(完整颗粒)小鼠单克隆抗体

关键功能

纯化、冻干

小鼠单克隆抗体

适用于斑点印迹、ELISA、ICC和中和测定

与AAV2、AAV2 7m8和AAV3完整颗粒反应

同种型:IgG3

Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG3
Conjugate Unconjugated
Application Affinity chromatography, Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification Affinity chromatography
Reactivity AAV2, AAV2 7m8, AAV3
No reactivity AAV1, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAVDJ, AAVrh10, AAVrh74
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution 2-8°C
Intended use Research use only
Clone A20
Immunogen AAV2 capsids
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Binding affinity

KD value (AAV2) = 2.6E-11 M

KD value (AAV3) = <1.0E-12 M

Synonym Adeno-associated virus 2; AAV-2

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Affinity Chromatography Assay dependent
Dot Blot 1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA Assay dependent
Immunoprecipitation (IP) 1:5
Neutralization Assay EC50 ~5 ng/ml (AAV2) and ~3 ng/ml (AAV3) – assay dependent

用于表征感染的不同阶段,对分析AAV2组装过程非常有用。A20与AAV2和AAV3、空衣壳和全衣壳特异性反应。识别组装衣壳的构象表位,不存在于变性衣壳蛋白和天然但未组装的衣壳蛋白中。该抗体不能用于免疫印迹。

表位定位实验(Wobus等人,2000)确定了四个免疫反应(不连续)区域。主要反应归因于AAV2衣壳的AA369至aa378序列。该抗体也可用于中和实验。

Wobus,C.E.等。针对腺相关病毒2型(AAV-2)衣壳的单克隆抗体:参与AAV-2细胞相互作用和中和AAV-2感染的衣壳结构域的表位定位和鉴定。J.维罗尔。74, 9281-93 (2000).

Moskalenko,M.等。人类抗腺相关病毒2型中和抗体的表位定位:对Ge的意义